6p5i

Electron Microscopy
3.1Å resolution

Structure of a mammalian 80S ribosome in complex with the Israeli Acute Paralysis Virus IRES (Class 1)

Released:

Function and Biology Details

Reaction catalysed:
The C-O-P bond 3' to the apurinic or apyrimidinic site in DNA is broken by a beta-elimination reaction, leaving a 3'-terminal unsaturated sugar and a product with a terminal 5'-phosphate
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero 81-mer (preferred)
PDBe Complex ID:
PDB-CPX-243878 (preferred)
Entry contents:
76 distinct polypeptide molecules
5 distinct RNA molecules
Macromolecules (81 distinct):
uS2 Chain: B
Molecule details ›
Chain: B
Length: 295 amino acids
Theoretical weight: 33 KDa
Source organism: Oryctolagus cuniculus
Small ribosomal subunit protein eS1 Chain: C
Molecule details ›
Chain: C
Length: 264 amino acids
Theoretical weight: 30 KDa
Source organism: Oryctolagus cuniculus
UniProt:
  • Canonical: G1SS70 (Residues: 1-264; Coverage: 100%)
Gene name: RPS3A
Sequence domains: Ribosomal S3Ae family
uS5 Chain: D
Small ribosomal subunit protein uS3 Chain: E
Small ribosomal subunit protein eS4 Chain: F
Small ribosomal subunit protein uS7 Chain: G
Small ribosomal subunit protein eS6 Chain: H
Small ribosomal subunit protein eS7 Chain: I
eS8 Chain: J
Small ribosomal subunit protein uS4 Chain: K
Small ribosomal subunit protein eS10 Chain: L
Small ribosomal subunit protein uS17 Chain: M
Small ribosomal subunit protein eS12 Chain: N
Small ribosomal subunit protein uS15 Chain: O
Small ribosomal subunit protein uS11 Chain: P
Small ribosomal subunit protein uS19 Chain: Q
Small ribosomal subunit protein uS9 Chain: R
Small ribosomal subunit protein eS17 Chain: S
Small ribosomal subunit protein uS13 Chain: T
Small ribosomal subunit protein eS19 Chain: U
Small ribosomal subunit protein uS10 Chain: V
Small ribosomal subunit protein eS21 Chain: W
Small ribosomal subunit protein uS8 Chain: X
Small ribosomal subunit protein uS12 Chain: Y
40S ribosomal protein S24 Chain: Z
Small ribosomal subunit protein eS25 Chain: a
Small ribosomal subunit protein eS26 Chain: b
Small ribosomal subunit protein eS27 Chain: c
Small ribosomal subunit protein eS28 Chain: d
Small ribosomal subunit protein uS14 Chain: e
Ubiquitin-like FUBI-ribosomal protein eS30 fusion protein Chain: f
Ubiquitin-ribosomal protein eS31 fusion protein Chain: g
Small ribosomal subunit protein RACK1 Chain: h
Large ribosomal subunit protein uL2 Chain: AA
Large ribosomal subunit protein uL3 Chain: AB
Large ribosomal subunit protein uL4 Chain: AC
Large ribosomal subunit protein uL18 Chain: AD
Large ribosomal subunit protein eL6 Chain: AE
Large ribosomal subunit protein uL30 Chain: AF
Large ribosomal subunit protein eL8 Chain: AG
Large ribosomal subunit protein uL6 Chain: AH
Large ribosomal subunit protein uL16 Chain: AI
Large ribosomal subunit protein uL5 Chain: AJ
eL13 Chain: AL
Large ribosomal subunit protein eL14 Chain: AM
Large ribosomal subunit protein eL15 Chain: AN
uL13 Chain: AO
Large ribosomal subunit protein uL22 Chain: AP
eL18 Chain: AQ
Ribosomal protein L19 Chain: AR
eL20 Chain: AS
Large ribosomal subunit protein eL21 Chain: AT
Large ribosomal subunit protein eL22 Chain: AU
Large ribosomal subunit protein uL14 Chain: AV
Large ribosomal subunit protein eL24 Chain: AW
Large ribosomal subunit protein uL23 Chain: AX
Large ribosomal subunit protein uL24 Chain: AY
Large ribosomal subunit protein eL27 Chain: AZ
Large ribosomal subunit protein uL15 Chain: Aa
Large ribosomal subunit protein eL29 Chain: Ab
Large ribosomal subunit protein eL30 Chain: Ac
Large ribosomal subunit protein eL31 Chain: Ad
Large ribosomal subunit protein eL32 Chain: Ae
Large ribosomal subunit protein eL33 Chain: Af
Large ribosomal subunit protein eL34 Chain: Ag
Large ribosomal subunit protein uL29 Chain: Ah
Large ribosomal subunit protein eL36 Chain: Ai
Large ribosomal subunit protein eL37 Chain: Aj
eL38 Chain: Ak
Large ribosomal subunit protein eL39 Chain: Al
eL40 Chain: Am
Small ribosomal subunit protein eS32 Chain: An
Large ribosomal subunit protein eL42 Chain: Ao
Large ribosomal subunit protein eL43 Chain: Ap
Large ribosomal subunit protein eL28 Chain: Ar
Large ribosomal subunit protein uL1 Chain: AK
18S Ribosomal RNA Chain: 2
IAPV-IRES Chain: 1
28S ribosomal RNA Chain: 5
5S ribosomal RNA Chain: 7
5.8S ribosomal RNA Chain: 8

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.1Å
Relevant EMDB volumes: EMD-20255