› 6p5t

X-ray diffraction
2.1Å resolution

Surface-layer (S-layer) RsaA protein from Caulobacter crescentus bound to strontium and iodide

Released:
DOI: 10.2210/pdb6p5t/pdb
PDB entry 6p5t coloured by chain, front view.
PDB entry 6p5t coloured by chain, side view.
PDB entry 6p5t coloured by chain, top view.
Source organism: Caulobacter vibrioides
Primary publication:
A bacterial surface layer protein exploits multistep crystallization for rapid self-assembly.
Herrmann J, Li PN, Jabbarpour F, Chan ACK, Rajkovic I, Matsui T, Shapiro L, Smit J, Weiss TM, Murphy MEP, Wakatsuki S
OpenAccess logo Proc Natl Acad Sci U S A 117 388-394 (2020)
PMID: 31848245

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-543451 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
S-layer protein Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 814 amino acids
Theoretical weight: 76.74 KDa
Source organism: Caulobacter vibrioides
Expression system: Escherichia coli
UniProt:
  • Canonical: P35828 (Residues: 223-1026; Coverage: 78%)
Gene names: CC_1007, rsaA
Sequence domains: RTX calcium-binding nonapeptide repeat (4 copies)

Ligands and Environments

2 bound ligands:
Ligand IOD 150 x IOD
Ligand SR 124 x SR
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CLSI BEAMLINE 08ID-1
Spacegroup: P21
Unit cell:
a: 210.729Å b: 80.564Å c: 221.833Å
α: 90° β: 117.44° γ: 90°
R-values:
R R work R free
0.207 0.206 0.243
Expression system: Escherichia coli

Quick links

Citations

1 review citation

S-Layer Ultrafiltration Membranes.
Schuster et al. (2021)
6 other articles

1 mention without citation

A bacterial surface layer protein exploits multistep crystallization for rapid self-assembly.
Herrmann et al. (2020)