› 6p7p

X-ray diffraction
1.66Å resolution

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
hetero dodecamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-505613 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct RNA molecule
Macromolecules (2 distinct):
Endodeoxyribonuclease NucC Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 243 amino acids
Theoretical weight: 26.9 KDa
Source organism: Escherichia coli MS 115-1
Expression system: Escherichia coli
UniProt:
  • Canonical: D7Y2H5 (Residues: 2-241; Coverage: 100%)
Gene names: HMPREF9540_01761, nucC
Sequence domains: Domain of unknown function (DUF6602)
Cyclic tri-AMP (5'-3' linked) Chains: D, E, F
Molecule details ›
Chains: D, E, F
Length: 3 nucleotides
Theoretical weight: 943 Da
Source organism: Escherichia coli MS 115-1
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand CL 1 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-E
Spacegroup: R32
Unit cell:
a: 131.592Å b: 131.592Å c: 252.109Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.143 0.142 0.162
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

22 review citations

Controlling and enhancing CRISPR systems.
Shivram et al. (2021)
21 more

2 mentions without citation

Bacterial origins of cyclic nucleotide-activated antiviral immune signaling.
Patel et al. (2022)
1 more