› 6p7q

X-ray diffraction
1.66Å resolution

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
hetero dodecamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-112894 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct RNA molecule
Macromolecules (2 distinct):
Endodeoxyribonuclease NucC Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 243 amino acids
Theoretical weight: 26.9 KDa
Source organism: Escherichia coli MS 115-1
Expression system: Escherichia coli
UniProt:
  • Canonical: D7Y2H5 (Residues: 2-241; Coverage: 100%)
Gene names: HMPREF9540_01761, nucC
Sequence domains: Domain of unknown function (DUF6602)
RNA (5'-R(P*AP*A)-3') Chains: D, E, F
Molecule details ›
Chains: D, E, F
Length: 2 nucleotides
Theoretical weight: 613 Da
Source organism: Escherichia coli MS 115-1
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand CL 1 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-E
Spacegroup: R32
Unit cell:
a: 131.781Å b: 131.781Å c: 252.836Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.16 0.159 0.185
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

24 review citations

Controlling and enhancing CRISPR systems.
Shivram et al. (2021)
23 more