› 6paq

X-ray diffraction
3.3Å resolution

Structure of a bacterial Atm1-family ABC exporter with ATP bound

Released:
DOI: 10.2210/pdb6paq/pdb
PDB entry 6paq coloured by chain, front view.
PDB entry 6paq coloured by chain, side view.
PDB entry 6paq coloured by chain, top view.
Source organism: Novosphingobium aromaticivorans DSM 12444
Primary publication:
A structural framework for unidirectional transport by a bacterial ABC exporter.
Fan C, Kaiser JT, Rees DC
OpenAccess logo Proc Natl Acad Sci U S A 117 19228-19236 (2020)
PMID: 32703810

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-557957 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
ATM1-type heavy metal exporter Chains: A, B
Molecule details ›
Chains: A, B
Length: 614 amino acids
Theoretical weight: 68.38 KDa
Source organism: Novosphingobium aromaticivorans DSM 12444
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q2G506 (Residues: 1-608; Coverage: 100%)
Gene names: Saro_2631, atm1
Sequence domains:

Ligands and Environments

1 bound ligand:
Ligand ATP 2 x ATP
1 modified residue:
Ligand MSE 26 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL12-2
Spacegroup: P212121
Unit cell:
a: 89.346Å b: 115.354Å c: 184.536Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.237 0.235 0.29
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

1 review citation

Interaction of Vanadium Complexes with Proteins: Revisiting the Reported Structures in the Protein Data Bank (PDB) since 2015.
Santos et al. (2023)
15 other articles

1 mention without citation

A structural framework for unidirectional transport by a bacterial ABC exporter.
Fan et al. (2020)