› 6pey

X-ray diffraction
2.88Å resolution

MTHFR with mutation Asp120Ala

Released:
DOI: 10.2210/pdb6pey/pdb
PDB entry 6pey coloured by chain, front view.
PDB entry 6pey coloured by chain, side view.
PDB entry 6pey coloured by chain, top view.
Source organism: Escherichia coli
Entry authors: Gallagher EL, Gurney LA, Frasco FG, Trimmer E, Bolen RL, Collins K, Garland E, Halloran J, Handley-Pendelton J, Hernandez V, Leffler S, Perez A, Soares A, Stojanoff V, Williams D

Function and Biology Details

Reaction catalysed: 
5-methyltetrahydrofolate + NAD(+) = 5,10-methylenetetrahydrofolate + NADH
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo dimer
Assembly name:
PDBe Complex ID:
PDB-CPX-142674 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
5,10-methylenetetrahydrofolate reductase Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 304 amino acids
Theoretical weight: 34.17 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P0AEZ1 (Residues: 1-296; Coverage: 100%)
Gene names: JW3913, b3941, metF
Sequence domains: Methylenetetrahydrofolate reductase

Ligands and Environments

1 bound ligand:
Ligand FAD 3 x FAD
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS-II BEAMLINE 17-ID-1
Spacegroup: C2
Unit cell:
a: 102.747Å b: 127.744Å c: 95.456Å
α: 90° β: 120.9° γ: 90°
R-values:
R R work R free
0.19 0.188 0.236
Expression system: Escherichia coli

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