› 6pfr

X-ray diffraction
1.51Å resolution

rsEGFP2 with a chlorinated chromophore in the fluorescent on-state

Released:
DOI: 10.2210/pdb6pfr/pdb
Model geometry
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PDB entry 6pfr coloured by chain, front view.
PDB entry 6pfr coloured by chain, side view.
PDB entry 6pfr coloured by chain, top view.
Source organism: Aequorea victoria
Primary publication:
Structural Evidence of Photoisomerization Pathways in Fluorescent Proteins.
Chang J, Romei MG, Boxer SG
J Am Chem Soc 141 15504-15508 (2019)
PMID: 31533429

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-154537 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Green fluorescent protein Chain: A
Molecule details ›
Chain: A
Length: 250 amino acids
Theoretical weight: 28.57 KDa
Source organism: Aequorea victoria
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P42212 (Residues: 1-238; Coverage: 100%)
Gene name: GFP
Sequence domains: Green fluorescent protein

Ligands and Environments

No bound ligands
1 modified residue:
Ligand OHD 1 x OHD

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: SSRL BEAMLINE BL12-2
Spacegroup: P212121
Unit cell:
a: 50.699Å b: 62.489Å c: 67.591Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.189 0.188 0.212
Expression system: Escherichia coli BL21(DE3)

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Citations

1 review citation

Photoswitchable Fluorescent Proteins: Mechanisms on Ultrafast Timescales.
Tang et al. (2022)
20 other articles

1 mention without citation

Structural and spectroscopic characterization of photoactive yellow protein and photoswitchable fluorescent protein constructs containing heavy atoms.
Romei et al. (2020)

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