› 6plm

X-ray diffraction
2.59Å resolution

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-144002 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Calmodulin-dependent glutamylase SidJ Chains: A, B
Molecule details ›
Chains: A, B
Length: 757 amino acids
Theoretical weight: 87.49 KDa
Source organism: Legionella pneumophila subsp. pneumophila str. Philadelphia 1
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q5ZTK6 (Residues: 97-853; Coverage: 87%)
Gene names: lpg2155, sidJ
Calmodulin-2 Chains: C, D
Molecule details ›
Chains: C, D
Length: 147 amino acids
Theoretical weight: 16.51 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0DP24 (Residues: 2-148; Coverage: 99%)
Gene names: CALM2, CAM2, CAMB
Sequence domains: EF-hand domain pair

Ligands and Environments

3 bound ligands:
Ligand CA 6 x CA
Ligand POP 2 x POP
Ligand AMP 2 x AMP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS-II BEAMLINE 17-ID-1
Spacegroup: P21
Unit cell:
a: 105.351Å b: 103.792Å c: 110.194Å
α: 90° β: 104.69° γ: 90°
R-values:
R R work R free
0.176 0.176 0.241
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

16 review citations

Pathogenicity and Virulence of Legionella: Intracellular replication and host response.
Chauhan et al. (2021)
15 more

2 mentions without citation

The unity of opposites: Strategic interplay between bacterial effectors to regulate cellular homeostasis.
Iyer et al. (2021)
1 more