6pod

Electron Microscopy
4.05Å resolution

ClpX-ClpP complex bound to substrate and ATP-gamma-S, class 2

Released:

Function and Biology Details

Reaction catalysed:
Hydrolysis of proteins to small peptides in the presence of ATP and magnesium. Alpha-Casein is the usual test substrate. In the absence of ATP, only oligopeptides shorter than five residues are hydrolyzed (such as succinyl-Leu-Tyr-|-NHMec; and Leu-Tyr-Leu-|-Tyr-Trp, in which cleavage of the -Tyr-|-Leu- and -Tyr-|-Trp bonds also occurs).
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero tetradecamer (preferred)
PDBe Complex ID:
PDB-CPX-141339 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
ATP-dependent Clp protease ATP-binding subunit ClpX Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 369 amino acids
Theoretical weight: 39.84 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
ATP-dependent Clp protease proteolytic subunit Chains: H, I, J, K, L, M, N
Molecule details ›
Chains: H, I, J, K, L, M, N
Length: 207 amino acids
Theoretical weight: 23.21 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
substrate peptide Chain: S

Ligands and Environments

2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 4.05Å
Relevant EMDB volumes: EMD-20412
Expression system: Escherichia coli