› 6ppr

Electron Microscopy
3.5Å resolution

Cryo-EM structure of AdnA(D934A)-AdnB(D1014A) in complex with AMPPNP and DNA

Released:
DOI: 10.2210/pdb6ppr/pdb
PDB entry 6ppr coloured by chain, front view.
PDB entry 6ppr coloured by chain, side view.
PDB entry 6ppr coloured by chain, top view.
Source organism: Mycolicibacterium smegmatis
Primary publication:
Structures and single-molecule analysis of bacterial motor nuclease AdnAB illuminate the mechanism of DNA double-strand break resection.
Jia N, Unciuleac MC, Xue C, Greene EC, Patel DJ, Shuman S
Proc Natl Acad Sci U S A 116 24507-24516 (2019)
PMID: 31740608
Related structures: EMD-20446

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-106317 (preferred)
Entry contents:
2 distinct polypeptide molecules
1 distinct DNA molecule
Macromolecules (3 distinct):
DNA 3'-5' helicase Chain: B
Molecule details ›
Chain: B
Length: 1095 amino acids
Theoretical weight: 118.08 KDa
Source organism: Mycolicibacterium smegmatis
Expression system: Escherichia coli
UniProt:
  • Canonical: I7FZ56 (Residues: 1-1095; Coverage: 100%)
Gene name: MSMEI_1900
Sequence domains:
DNA 3'-5' helicase Chain: A
Molecule details ›
Chain: A
Length: 1045 amino acids
Theoretical weight: 110.9 KDa
Source organism: Mycolicibacterium smegmatis
Expression system: Escherichia coli
UniProt:
  • Canonical: A0QTR9 (Residues: 1-1045; Coverage: 100%)
Gene name: MSMEG_1941
Sequence domains:
DNA (70-MER) Chain: X
Molecule details ›
Chain: X
Length: 70 nucleotides
Theoretical weight: 21.48 KDa

Ligands and Environments

2 bound ligands:
Ligand ANP 1 x ANP
Ligand SF4 1 x SF4
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.5Å
Relevant EMDB volumes: EMD-20446
Expression system: Escherichia coli

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Citations

3 review citations

Bacterial phenotypic heterogeneity in DNA repair and mutagenesis.
Vincent et al. (2020)
2 more

3 mentions without citation

Recent Insights into the Structure and Function of Mycobacterial Membrane Proteins Facilitated by Cryo-EM.
Bendre et al. (2021)
2 more