› 6psy

Electron Microscopy
2.8Å resolution

Cryo-EM structure of S. cerevisiae Drs2p-Cdc50p in the autoinhibited apo form

Released:
DOI: 10.2210/pdb6psy/pdb
PDB entry 6psy coloured by chain, front view.
PDB entry 6psy coloured by chain, side view.
PDB entry 6psy coloured by chain, top view.
Source organism: Saccharomyces cerevisiae W303
Primary publication:
Autoinhibition and activation mechanisms of the eukaryotic lipid flippase Drs2p-Cdc50p.
Bai L, Kovach A, You Q, Hsu HC, Zhao G, Li H
OpenAccess logo Nat Commun 10 4142 (2019)
PMID: 31515475
Related structures: EMD-20468

Function and Biology Details

Reaction catalysed: 
ATP + H(2)O + phospholipid(Side 1) = ADP + phosphate + phospholipid(Side 2)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-150549 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (3 distinct):
Phospholipid-transporting ATPase DRS2 Chain: A
Molecule details ›
Chain: A
Length: 1355 amino acids
Theoretical weight: 153.93 KDa
Source organism: Saccharomyces cerevisiae W303
Expression system: Saccharomyces cerevisiae W303
UniProt:
  • Canonical: P39524 (Residues: 1-1355; Coverage: 100%)
Gene names: DRS2, FUN38, SWA3, YAL026C
Sequence domains:
Phospholipid-transporting ATPase accessory subunit CDC50 Chain: E
Molecule details ›
Chain: E
Length: 391 amino acids
Theoretical weight: 45.04 KDa
Source organism: Saccharomyces cerevisiae W303
Expression system: Saccharomyces cerevisiae W303
UniProt:
  • Canonical: P25656 (Residues: 1-391; Coverage: 100%)
Gene names: CDC50, SWA4, YCR094W, YCR94W
Sequence domains: LEM3 (ligand-effect modulator 3) family / CDC50 family

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
1 bound ligand:
Ligand NAG 2 x NAG
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 2.8Å
Relevant EMDB volumes: EMD-20468
Expression system: Saccharomyces cerevisiae W303

Quick links

Citations

7 review citations

Novel roles of phosphoinositides in signaling, lipid transport, and disease.
Hammond et al. (2020)
6 more

3 mentions without citation

Consensus, controversies, and conundrums of P4-ATPases: The emerging face of eukaryotic lipid flippases.
Duan et al. (2024)
2 more