› 6pwg

X-ray diffraction
1.89Å resolution

Ewingella americana HopBF1 kinase bound to AMP-PNP

Released:
DOI: 10.2210/pdb6pwg/pdb
PDB entry 6pwg coloured by chain, front view.
PDB entry 6pwg coloured by chain, side view.
PDB entry 6pwg coloured by chain, top view.
Source organism: Ewingella americana
Primary publication:
A Bacterial Effector Mimics a Host HSP90 Client to Undermine Immunity.
Lopez VA, Park BC, Nowak D, Sreelatha A, Zembek P, Fernandez J, Servage KA, Gradowski M, Hennig J, Tomchick DR, Pawłowski K, Krzymowska M, Tagliabracci VS
Cell 179 205-218.e21 (2019)
PMID: 31522888

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-100576 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Type III effector HopBF1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 204 amino acids
Theoretical weight: 22.6 KDa
Source organism: Ewingella americana
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A085GHR3 (Residues: 1-203; Coverage: 100%)
Gene name: GEAM_1328

Ligands and Environments

2 bound ligands:
Ligand ANP 2 x ANP
Ligand EDO 5 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: R32
Unit cell:
a: 76.185Å b: 76.185Å c: 351.362Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.208 0.206 0.241
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

13 review citations

Post-translational modifications of Hsp90 and translating the chaperone code.
Backe et al. (2020)
12 more

1 mention without citation

A Bacterial Effector Mimics a Host HSP90 Client to Undermine Immunity.
Lopez et al. (2019)