› 6pwm

X-ray diffraction
2.4Å resolution

ADC-7 in complex with Beta-lactam antibiotic ceftazidime

Released:
DOI: 10.2210/pdb6pwm/pdb
PDB entry 6pwm coloured by chain, front view.
PDB entry 6pwm coloured by chain, side view.
PDB entry 6pwm coloured by chain, top view.
Source organism: Acinetobacter baumannii
Primary publication:
Structural Insights into Inhibition of the Acinetobacter-Derived Cephalosporinase ADC-7 by Ceftazidime and Its Boronic Acid Transition State Analog.
Curtis BN, Smolen KA, Barlow SJ, Caselli E, Prati F, Taracila MA, Bonomo RA, Wallar BJ, Powers RA
Antimicrob Agents Chemother 64 (2020)
PMID: 32988830

Function and Biology Details

Reaction catalysed: 
A beta-lactam + H(2)O = a substituted beta-amino acid
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-179554 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Beta-lactamase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 361 amino acids
Theoretical weight: 40.81 KDa
Source organism: Acinetobacter baumannii
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q6DRA1 (Residues: 24-383; Coverage: 100%)
Sequence domains: Beta-lactamase

Ligands and Environments

2 bound ligands:
Ligand CAZ 4 x CAZ
Ligand GLY 1 x GLY
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-G
Spacegroup: P21
Unit cell:
a: 92.261Å b: 81.661Å c: 105.22Å
α: 90° β: 112.61° γ: 90°
R-values:
R R work R free
0.216 0.214 0.254
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

1 review citation

Class C β-Lactamases: Molecular Characteristics.
Philippon et al. (2022)
3 other articles

2 mentions without citation

Structural Characterization of the D179N and D179Y Variants of KPC-2 β-Lactamase: Ω-Loop Destabilization as a Mechanism of Resistance to Ceftazidime-Avibactam.
Alsenani et al. (2022)
1 more