› 6py4

X-ray diffraction
2Å resolution

Crystal structure of ligand-binding domain of Pseudomonas fluorescens chemoreceptor CtaA in complex with L-leucine

Released:
DOI: 10.2210/pdb6py4/pdb
PDB entry 6py4 coloured by chain, front view.
PDB entry 6py4 coloured by chain, side view.
PDB entry 6py4 coloured by chain, top view.
Source organism: Pseudomonas fluorescens Pf0-1
Primary publication:
Broad Specificity of Amino Acid Chemoreceptor CtaA of Pseudomonas fluorescens Is Afforded by Plasticity of Its Amphipathic Ligand-Binding Pocket.
Ud-Din AIMS, Khan MF, Roujeinikova A
Mol Plant Microbe Interact 33 612-623 (2020)
PMID: 31909676

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-174703 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Putative methyl-accepting chemotaxis protein Chain: A
Molecule details ›
Chain: A
Length: 250 amino acids
Theoretical weight: 27.06 KDa
Source organism: Pseudomonas fluorescens Pf0-1
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q3K7T6 (Residues: 34-277; Coverage: 39%)
Gene name: Pfl01_4431
Sequence domains: Cache domain

Ligands and Environments

1 bound ligand:
Ligand LEU 1 x LEU
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: AUSTRALIAN SYNCHROTRON BEAMLINE MX1
Spacegroup: I212121
Unit cell:
a: 70.21Å b: 71.44Å c: 112.77Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.193 0.19 0.238
Expression system: Escherichia coli BL21(DE3)

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Citations

8 citation in other articles

The dCache Chemoreceptor TlpA of Helicobacter pylori Binds Multiple Attractant and Antagonistic Ligands via Distinct Sites.
Johnson et al. (2021)
7 more