› 6q1s

X-ray diffraction
2.3Å resolution

A hypothetical aminotransferase from Mycobacterium tuberculosis, alpha-ketoglutarate and PMP bound form

Released:
DOI: 10.2210/pdb6q1s/pdb
PDB entry 6q1s coloured by chain, front view.
PDB entry 6q1s coloured by chain, side view.
PDB entry 6q1s coloured by chain, top view.
Source organism: Mycobacterium tuberculosis H37Rv
Entry authors: Duan L, Sacchettini J

Function and Biology Details

Reactions catalysed: 
D-alanine + 2-oxoglutarate = pyruvate + D-glutamate
4-amino-4-deoxychorismate = 4-aminobenzoate + pyruvate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-181261 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Bifunctional aminodeoxychorismate lyase / D-amino acid transaminase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 291 amino acids
Theoretical weight: 31.34 KDa
Source organism: Mycobacterium tuberculosis H37Rv
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q79FW0 (Residues: 1-289; Coverage: 100%)
Gene name: Rv0812
Sequence domains: Amino-transferase class IV

Ligands and Environments


Cofactor: Ligand PMP 4 x PMP
2 bound ligands:
Ligand AKG 4 x AKG
Ligand CIT 2 x CIT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-D
Spacegroup: P21
Unit cell:
a: 66.545Å b: 202.257Å c: 48.121Å
α: 90° β: 90.05° γ: 90°
R-values:
R R work R free
0.215 0.205 0.227
Expression system: Escherichia coli BL21(DE3)

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