› 6q6g

Electron Microscopy
3.2Å resolution

Cryo-EM structure of the APC/C-Cdc20-Cdk2-cyclinA2-Cks2 complex, the D1 box class

Released:
DOI: 10.2210/pdb6q6g/pdb
PDB entry 6q6g coloured by chain, front view.
PDB entry 6q6g coloured by chain, side view.
PDB entry 6q6g coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Cyclin A2 degradation during the spindle assembly checkpoint requires multiple binding modes to the APC/C.
Zhang S, Tischer T, Barford D
OpenAccess logo Nat Commun 10 3863 (2019)
PMID: 31455778
Related structures: EMD-4465

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero 21-mer (preferred)
PDBe Complex ID:
PDB-CPX-148907 (preferred)
Entry contents:
16 distinct polypeptide molecules
Macromolecules (16 distinct):
Cell division cycle protein 20 homolog Chain: R
Molecule details ›
Chain: R
Length: 499 amino acids
Theoretical weight: 54.8 KDa
Source organism: Homo sapiens
Expression system: unidentified baculovirus
UniProt:
  • Canonical: Q12834 (Residues: 1-499; Coverage: 100%)
Gene name: CDC20
Sequence domains:
Cyclin-A2 Chain: S
Molecule details ›
Chain: S
Length: 400 amino acids
Theoretical weight: 44.94 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P20248 (Residues: 1-432; Coverage: 93%)
Gene names: CCN1, CCNA, CCNA2
Sequence domains:
Anaphase-promoting complex subunit 10 Chain: L
Anaphase-promoting complex subunit 15 Chain: D
Anaphase-promoting complex subunit 1 Chain: A
Anaphase-promoting complex subunit 2 Chain: N
Anaphase-promoting complex subunit 4 Chain: I
Anaphase-promoting complex subunit 5 Chain: O
Cell division cycle protein 16 homolog Chains: K, Q
Anaphase-promoting complex subunit 11 Chain: C
Anaphase-promoting complex subunit CDC26 Chains: G, W
Anaphase-promoting complex subunit 13 Chain: M
Anaphase-promoting complex subunit 16 Chain: H
Cell division cycle protein 27 homolog Chains: J, P
Anaphase-promoting complex subunit 7 Chains: Y, Z
Cell division cycle protein 23 homolog Chains: U, V

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this EM entry.
Resolution: 3.2Å
Relevant EMDB volumes: EMD-4465
Expression systems:
  • unidentified baculovirus
  • Escherichia coli

Quick links

Citations

6 review citations

APC/C ubiquitin ligase: Functions and mechanisms in tumorigenesis.
Schrock et al. (2020)
5 more

4 mentions without citation

Using Budding Yeast to Identify Molecules That Block Cancer Cell 'Mitotic Slippage' Only in the Presence of Mitotic Poisons.
Schuyler et al. (2021)
3 more