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Structures of Respiratory Supercomplex I+III2 Reveal Functional and Conformational Crosstalk.
<div class='caption-title'>Purification of SC I+III2 from Ovine Mitochondria</div>
<div class='caption-title'>NADH:cyt c Oxidoreductase Activity of Isolated SC I+III2</div>
Letts JA, Fiedorczuk K, Degliesposti G, Skehel M, Sazanov LA
OpenAccess logo Mol Cell 75 1131-1146.e6 (2019)
Related entries: 6q9b, 6q9e, 6qa9, 6qbx, 6qc2, 6qc3, 6qc4, 6qc5, 6qc6, 6qc7, 6qc8, 6qc9, 6qca, 6qcf

Cited: 87 times
EuropePMC logo PMID: 31492636

Abstract

The mitochondrial electron transport chain complexes are organized into supercomplexes (SCs) of defined stoichiometry, which have been proposed to regulate electron flux via substrate channeling. We demonstrate that CoQ trapping in the isolated SC I+III2 limits complex (C)I turnover, arguing against channeling. The SC structure, resolved at up to 3.8 Å in four distinct states, suggests that CoQ oxidation may be rate limiting because of unequal access of CoQ to the active sites of CIII2. CI shows a transition between "closed" and "open" conformations, accompanied by the striking rotation of a key transmembrane helix. Furthermore, the state of CI affects the conformational flexibility within CIII2, demonstrating crosstalk between the enzymes. CoQ was identified at only three of the four binding sites in CIII2, suggesting that interaction with CI disrupts CIII2 symmetry in a functionally relevant manner. Together, these observations indicate a more nuanced functional role for the SCs.

Articles - 6q9d mentioned but not cited (2)

  1. Structures of Respiratory Supercomplex I+III2 Reveal Functional and Conformational Crosstalk. Letts JA, Fiedorczuk K, Degliesposti G, Skehel M, Sazanov LA. Mol Cell 75 1131-1146.e6 (2019)
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Reviews citing this publication (23)

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