› 6qfs

X-ray diffraction
2.2Å resolution

Chargeless variant of the Cellulose-binding domain from Cellulomonas fimi

Released:
DOI: 10.2210/pdb6qfs/pdb
PDB entry 6qfs coloured by chain, front view.
PDB entry 6qfs coloured by chain, side view.
PDB entry 6qfs coloured by chain, top view.
Source organism: Cellulomonas fimi
Primary publication:
Charge Interactions in a Highly Charge-depleted Protein
Hervo-Hansen S, Hojgaard C, Johansson K, Wang Y, Wahni K, Young D, Messens J, Teilum K, Lindorff-Larsen K, Winther JR
J. Am. Chem. Soc. (2021)

Function and Biology Details

Reactions catalysed: 
Endohydrolysis of (1->4)-beta-D-xylosidic linkages in xylans
Hydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose and cellotetraose, releasing cellobiose from the non-reducing ends of the chains
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-139868 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Exoglucanase/xylanase Chains: A, B, C, D, E, F, G, H
Molecule details ›
Chains: A, B, C, D, E, F, G, H
Length: 106 amino acids
Theoretical weight: 10.79 KDa
Source organism: Cellulomonas fimi
Expression system: Escherichia coli
UniProt:
  • Canonical: P07986 (Residues: 380-484; Coverage: 24%)
Gene names: cex, xynB
Sequence domains: Cellulose binding domain

Ligands and Environments

7 bound ligands:
Ligand ACT 8 x ACT
Ligand EDO 16 x EDO
Ligand FMT 25 x FMT
Ligand 1PS 4 x 1PS
Ligand PEG 2 x PEG
Ligand ETE 2 x ETE
Ligand GOL 1 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID30B
Spacegroup: P3221
Unit cell:
a: 137.382Å b: 137.382Å c: 148.548Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.178 0.177 0.202
Expression system: Escherichia coli

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