› 6qg6

Electron Microscopy
10.4Å resolution

Structure of eIF2B-eIF2 (phosphorylated at Ser51) complex (model D)

Released:
DOI: 10.2210/pdb6qg6/pdb
PDB entry 6qg6 coloured by chain, front view.
PDB entry 6qg6 coloured by chain, side view.
PDB entry 6qg6 coloured by chain, top view.
Source organism: Saccharomyces cerevisiae
Primary publication:
Structural basis for the inhibition of translation through eIF2α phosphorylation.
Gordiyenko Y, Llácer JL, Ramakrishnan V
OpenAccess logo Nat Commun 10 2640 (2019)
PMID: 31201334
Related structures: EMD-4548

Function and Biology Details

Reaction catalysed: 
GTP + H(2)O = GDP + phosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero hexadecamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-140532 (preferred)
Entry contents:
8 distinct polypeptide molecules
Macromolecules (8 distinct):
Translation initiation factor eIF2B subunit alpha Chains: A, B
Molecule details ›
Chains: A, B
Length: 305 amino acids
Theoretical weight: 34.06 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Saccharomyces cerevisiae
UniProt:
  • Canonical: P14741 (Residues: 1-305; Coverage: 100%)
Gene names: AAS2, GCN3, TIF221, YKR026C
Sequence domains: Initiation factor 2 subunit family
Translation initiation factor eIF2B subunit beta Chains: C, D
Molecule details ›
Chains: C, D
Length: 381 amino acids
Theoretical weight: 42.62 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Saccharomyces cerevisiae
UniProt:
  • Canonical: P32502 (Residues: 1-381; Coverage: 100%)
Gene names: GCD7, L8003.17, TIF222, YLR291C
Sequence domains: Initiation factor 2 subunit family
Translation initiation factor eIF2B subunit gamma Chains: E, F
Molecule details ›
Chains: E, F
Length: 578 amino acids
Theoretical weight: 65.77 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Saccharomyces cerevisiae
UniProt:
  • Canonical: P09032 (Residues: 1-578; Coverage: 100%)
Gene names: GCD1, TIF223, TRA3, YOR260W
Translation initiation factor eIF2B subunit delta Chains: G, H
Molecule details ›
Chains: G, H
Length: 651 amino acids
Theoretical weight: 70.95 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Saccharomyces cerevisiae
UniProt:
  • Canonical: P12754 (Residues: 1-651; Coverage: 100%)
Gene names: GCD2, TIF224, YGR083C
Sequence domains: Initiation factor 2 subunit family
Translation initiation factor eIF2B subunit epsilon Chains: I, J
Molecule details ›
Chains: I, J
Length: 712 amino acids
Theoretical weight: 81.25 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Saccharomyces cerevisiae
UniProt:
  • Canonical: P32501 (Residues: 1-712; Coverage: 100%)
Gene names: GCD6, TIF225, YD8142.12, YD8142B.03, YDR211W
Sequence domains:
Eukaryotic translation initiation factor 2 subunit alpha Chains: K, L
Molecule details ›
Chains: K, L
Length: 304 amino acids
Theoretical weight: 34.84 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Saccharomyces cerevisiae
UniProt:
  • Canonical: P20459 (Residues: 1-304; Coverage: 100%)
Gene names: J1429, SUI2, TIF211, YJR007W
Sequence domains:
Eukaryotic translation initiation factor 2 subunit gamma Chains: M, N
Molecule details ›
Chains: M, N
Length: 527 amino acids
Theoretical weight: 57.94 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Saccharomyces cerevisiae
UniProt:
  • Canonical: P32481 (Residues: 1-527; Coverage: 100%)
Gene names: GCD11, SUI4, TIF213, YER025W
Sequence domains:
Eukaryotic translation initiation factor 2 subunit beta Chains: O, P
Molecule details ›
Chains: O, P
Length: 285 amino acids
Theoretical weight: 31.63 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Saccharomyces cerevisiae
UniProt:
  • Canonical: P09064 (Residues: 1-285; Coverage: 100%)
Gene names: SUI3, TIF212, YPL237W
Sequence domains: Domain found in IF2B/IF5

Ligands and Environments

No bound ligands
1 modified residue:
Ligand SEP 2 x SEP

Experiments and Validation Details

Entry percentile scores
Resolution: 10.4Å
Relevant EMDB volumes: EMD-4548
Expression system: Saccharomyces cerevisiae

Quick links

Citations

17 review citations

The integrated stress response: From mechanism to disease.
Costa-Mattioli et al. (2020)
16 more

1 mention without citation

VESPER: global and local cryo-EM map alignment using local density vectors.
Han et al. (2021)