› 6qkj

X-ray diffraction
2.2Å resolution

EgtB from Chloracidobacterium thermophilum, a type II sulfoxide synthase in complex with N,N,N-trimethyl-histidine

Released:
DOI: 10.2210/pdb6qkj/pdb
PDB entry 6qkj coloured by chain, front view.
PDB entry 6qkj coloured by chain, side view.
PDB entry 6qkj coloured by chain, top view.
Source organism: Chloracidobacterium thermophilum B
Primary publication:
An Alternative Active Site Architecture for O2 Activation in the Ergothioneine Biosynthetic EgtB from Chloracidobacterium thermophilum.
Stampfli AR, Goncharenko KV, Meury M, Dubey BN, Schirmer T, Seebeck FP
J Am Chem Soc 141 5275-5285 (2019)
PMID: 30883103

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-124263 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ergothioneine biosynthesis protein EgtB Chains: A, B
Molecule details ›
Chains: A, B
Length: 437 amino acids
Theoretical weight: 49.44 KDa
Source organism: Chloracidobacterium thermophilum B
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: G2LET6 (Residues: 1-434; Coverage: 100%)
Gene name: Cabther_A1318
Sequence domains:

Ligands and Environments

4 bound ligands:
Ligand FE 4 x FE
Ligand AVJ 2 x AVJ
Ligand CL 4 x CL
Ligand IMD 2 x IMD
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06DA
Spacegroup: C2221
Unit cell:
a: 108.143Å b: 201.288Å c: 108.332Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.197 0.195 0.238
Expression system: Escherichia coli BL21(DE3)

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Citations

2 review citations

Chemical modifications of proteins and their applications in metalloenzyme studies.
Naowarojna et al. (2021)
1 more