› 6qlg

X-ray diffraction
2.15Å resolution

Crystal structure of AnUbiX (PadA1) in complex with FMN and dimethylallyl pyrophosphate

Released:
DOI: 10.2210/pdb6qlg/pdb
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PDB entry 6qlg coloured by chain, front view.
PDB entry 6qlg coloured by chain, side view.
PDB entry 6qlg coloured by chain, top view.
Source organism: Aspergillus niger
Primary publication:
The UbiX flavin prenyltransferase reaction mechanism resembles class I terpene cyclase chemistry.
Marshall SA, Payne KAP, Fisher K, White MD, Ní Cheallaigh A, Balaikaite A, Rigby SEJ, Leys D
OpenAccess logo Nat Commun 10 2357 (2019)
PMID: 31142738

Function and Biology Details

Reaction catalysed: 
Prenyl phosphate + FMNH(2) = prenylated FMNH(2) + phosphate
Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dodecamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-107113 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Flavin prenyltransferase PAD1, mitochondrial Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 227 amino acids
Theoretical weight: 25.08 KDa
Source organism: Aspergillus niger
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A3F715 (Residues: 1-227; Coverage: 100%)
Gene names: An03g06570, PAD1
Sequence domains: Flavoprotein

Ligands and Environments


Cofactor: Ligand FMN 6 x FMN
3 bound ligands:
Ligand DMA 6 x DMA
Ligand PO4 1 x PO4
Ligand PEG 2 x PEG
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: DIAMOND BEAMLINE I03
Spacegroup: P43212
Unit cell:
a: 102Å b: 102Å c: 276.69Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.162 0.161 0.182
Expression system: Escherichia coli BL21(DE3)

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Citations

5 review citations

Bacterial terpenome.
Rudolf et al. (2021)
4 more

1 mention without citation

The UbiX flavin prenyltransferase reaction mechanism resembles class I terpene cyclase chemistry.
Marshall et al. (2019)

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