› 6qq8

X-ray diffraction
1.46Å resolution

Cryogenic temperature structure of the fluorescent protein Cerulean recorded after an accumulated dose of 290 kGy

Released:
DOI: 10.2210/pdb6qq8/pdb
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PDB entry 6qq8 coloured by chain, front view.
PDB entry 6qq8 coloured by chain, side view.
PDB entry 6qq8 coloured by chain, top view.
Source organism: Aequorea victoria
Primary publication:
Specific radiation damage is a lesser concern at room temperature.
Gotthard G, Aumonier S, De Sanctis D, Leonard G, von Stetten D, Royant A
OpenAccess logo IUCrJ 6 665-680 (2019)
PMID: 31316810

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-154537 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Green fluorescent protein Chain: A
Molecule details ›
Chain: A
Length: 237 amino acids
Theoretical weight: 26.79 KDa
Source organism: Aequorea victoria
Expression system: Escherichia coli
UniProt:
  • Canonical: P42212 (Residues: 2-238; Coverage: 100%)
Gene name: GFP
Sequence domains: Green fluorescent protein

Ligands and Environments

No bound ligands
1 modified residue:
Ligand CRF 1 x CRF

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: ESRF BEAMLINE ID29
Spacegroup: P212121
Unit cell:
a: 51.11Å b: 62.72Å c: 70.34Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.165 0.163 0.197
Expression system: Escherichia coli

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Citations

1 review citation

Best practices for time-resolved serial synchrotron crystallography.
Schulz et al. (2022)
26 other articles

1 mention without citation

Specific radiation damage is a lesser concern at room temperature.
Gotthard et al. (2019)

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