› 6qrl

X-ray diffraction
1.84Å resolution

Crystal structure of ShkA _Rec1 in complex with c-di-GMP

Released:
DOI: 10.2210/pdb6qrl/pdb
PDB entry 6qrl coloured by chain, front view.
PDB entry 6qrl coloured by chain, side view.
PDB entry 6qrl coloured by chain, top view.
Source organism: Caulobacter vibrioides CB15
Primary publication:
Hybrid histidine kinase activation by cyclic di-GMP-mediated domain liberation.
Dubey BN, Agustoni E, Böhm R, Kaczmarczyk A, Mangia F, von Arx C, Jenal U, Hiller S, Plaza-Menacho I, Schirmer T
OpenAccess logo Proc Natl Acad Sci U S A 117 1000-1008 (2020)
PMID: 31882446

Function and Biology Details

Reaction catalysed: 
ATP + protein L-histidine = ADP + protein N-phospho-L-histidine
Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-573310 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
histidine kinase Chains: A, B
Molecule details ›
Chains: A, B
Length: 130 amino acids
Theoretical weight: 13.24 KDa
Source organism: Caulobacter vibrioides CB15
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9ABT2 (Residues: 243-372; Coverage: 25%)
Gene names: CC0138, CC_0138, shkA

Ligands and Environments

2 bound ligands:
Ligand C2E 4 x C2E
Ligand SO4 2 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06DA
Spacegroup: P3221
Unit cell:
a: 55.11Å b: 55.11Å c: 179.661Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.209 0.207 0.24
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

1 review citation

Polarity of c-di-GMP synthesis and degradation.
Kreiling et al. (2023)
10 other articles

4 mentions without citation

Diversity in Sensing and Signaling of Bacterial Sensor Histidine Kinases.
Ishii et al. (2021)
3 more