6qtu Citations

Plant photoreceptors and their signaling components compete for COP1 binding via VP peptide motifs.

OpenAccess logo EMBO J 38 e102140 (2019)
Related entries: 6qto, 6qtq, 6qtr, 6qts, 6qtt, 6qtv, 6qtw, 6qtx

Cited: 60 times
EuropePMC logo PMID: 31304983

Abstract

Plants sense different parts of the sun's light spectrum using distinct photoreceptors, which signal through the E3 ubiquitin ligase COP1. Here, we analyze why many COP1-interacting transcription factors and photoreceptors harbor sequence-divergent Val-Pro (VP) motifs that bind COP1 with different binding affinities. Crystal structures of the VP motifs of the UV-B photoreceptor UVR8 and the transcription factor HY5 in complex with COP1, quantitative binding assays, and reverse genetic experiments together suggest that UVR8 and HY5 compete for COP1. Photoactivation of UVR8 leads to high-affinity cooperative binding of its VP motif and its photosensing core to COP1, preventing COP1 binding to its substrate HY5. UVR8-VP motif chimeras suggest that UV-B signaling specificity resides in the UVR8 photoreceptor core. Different COP1-VP peptide motif complexes highlight sequence fingerprints required for COP1 targeting. The blue-light photoreceptors CRY1 and CRY2 also compete with transcription factors for COP1 binding using similar VP motifs. Thus, our work reveals that different photoreceptors and their signaling components compete for COP1 via a conserved mechanism to control different light signaling cascades.

Reviews citing this publication (14)

  1. The Photomorphogenic Central Repressor COP1: Conservation and Functional Diversification during Evolution. Han X, Huang X, Deng XW. Plant Commun 1 100044 (2020)
  2. HY5: A Pivotal Regulator of Light-Dependent Development in Higher Plants. Xiao Y, Chu L, Zhang Y, Bian Y, Xiao J, Xu D. Front Plant Sci 12 800989 (2021)
  3. Illuminating the COP1/SPA Ubiquitin Ligase: Fresh Insights Into Its Structure and Functions During Plant Photomorphogenesis. Ponnu J, Hoecker U. Front Plant Sci 12 662793 (2021)
  4. Signaling Mechanisms by Arabidopsis Cryptochromes. Ponnu J, Hoecker U. Front Plant Sci 13 844714 (2022)
  5. Cryptochromes and the Circadian Clock: The Story of a Very Complex Relationship in a Spinning World. Lopez L, Fasano C, Perrella G, Facella P. Genes (Basel) 12 672 (2021)
  6. Integration of Light and Brassinosteroid Signaling during Seedling Establishment. Lin F, Cao J, Yuan J, Liang Y, Li J. Int J Mol Sci 22 12971 (2021)
  7. Plant responses to UV-B radiation: signaling, acclimation and stress tolerance. Chen Z, Dong Y, Huang X. Stress Biol 2 51 (2022)
  8. Light signaling in plants-a selective history. Huq E, Lin C, Quail PH. Plant Physiol 195 213-231 (2024)
  9. The involvement of the N-terminal PHR domain of Arabidopsis cryptochromes in mediating light signaling. Wang W, Mao Z, Guo T, Kou S, Yang HQ. aBIOTECH 2 146-155 (2021)
  10. The lowdown on breakdown: Open questions in plant proteolysis. Eckardt NA, Avin-Wittenberg T, Bassham DC, Chen P, Chen Q, Fang J, Genschik P, Ghifari AS, Guercio AM, Gibbs DJ, Heese M, Jarvis RP, Michaeli S, Murcha MW, Mursalimov S, Noir S, Palayam M, Peixoto B, Rodriguez PL, Schaller A, Schnittger A, Serino G, Shabek N, Stintzi A, Theodoulou FL, Üstün S, van Wijk KJ, Wei N, Xie Q, Yu F, Zhang H. Plant Cell 36 2931-2975 (2024)
  11. Unique and contrasting effects of light and temperature cues on plant transcriptional programs. Jarad M, Antoniou-Kourounioti R, Hepworth J, Qüesta JI. Transcription 11 134-159 (2020)
  12. Creativity comes from interactions: modules of protein interactions in plants. Allen JR, Wilkinson EG, Strader LC. FEBS J 289 1492-1514 (2022)
  13. Label-free biomolecular and cellular methods in small molecule epigallocatechin-gallate research. Péter B, Szekacs I, Horvath R. Heliyon 10 e25603 (2024)
  14. Optogenetic tools controlled by ultraviolet-B light. Ouyang X, Ren H, Huang X. aBIOTECH 2 170-175 (2021)

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