› 6qw1

X-ray diffraction
1.84Å resolution

The Transcriptional Regulator PrfA-L140F mutant from Listeria Monocytogenes

Released:
DOI: 10.2210/pdb6qw1/pdb
PDB entry 6qw1 coloured by chain, front view.
PDB entry 6qw1 coloured by chain, side view.
PDB entry 6qw1 coloured by chain, top view.
Source organism: Listeria monocytogenes
Primary publication:
A Novel Growth-Based Selection Strategy Identifies New Constitutively Active Variants of the Major Virulence Regulator PrfA in Listeria monocytogenes.
Hansen S, Hall M, Grundström C, Brännström K, Sauer-Eriksson AE, Johansson J
OpenAccess logo J Bacteriol 202 (2020)
PMID: 32179627

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-149566 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Listeriolysin regulatory protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 239 amino acids
Theoretical weight: 27.49 KDa
Source organism: Listeria monocytogenes
Expression system: Escherichia coli
UniProt:
  • Canonical: P22262 (Residues: 1-237; Coverage: 100%)
Gene names: lmo0200, prfA

Ligands and Environments

2 bound ligands:
Ligand IPA 7 x IPA
Ligand NA 3 x NA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID30B
Spacegroup: P212121
Unit cell:
a: 47.634Å b: 88.806Å c: 115.439Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.183 0.181 0.241
Expression system: Escherichia coli

Quick links

Citations

1 citations in other articles

NrnA Is a Linear Dinucleotide Phosphodiesterase with Limited Function in Cyclic Dinucleotide Metabolism in Listeria monocytogenes.
Gall et al. (2022)

1 mention without citation

A Novel Growth-Based Selection Strategy Identifies New Constitutively Active Variants of the Major Virulence Regulator PrfA in Listeria monocytogenes.
Hansen et al. (2020)