› 6qw4

X-ray diffraction
2.1Å resolution

Engineered streptavidin variant (ACGR) in complex with the Strep-tag II peptide

Released:
DOI: 10.2210/pdb6qw4/pdb
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PDB entry 6qw4 coloured by chain, front view.
PDB entry 6qw4 coloured by chain, side view.
PDB entry 6qw4 coloured by chain, top view.
Source organisms:
Primary publication:
The Role of Changing Loop Conformations in Streptavidin Versions Engineered for High-affinity Binding of the Strep-tag II Peptide.
Schmidt TGM, Eichinger A, Schneider M, Bonet L, Carl U, Karthaus D, Theobald I, Skerra A
J Mol Biol 433 166893 (2021)
PMID: 33639211

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero octamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-149738 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Streptavidin Chain: A
Molecule details ›
Chain: A
Length: 127 amino acids
Theoretical weight: 13.36 KDa
Source organism: Streptomyces avidinii
Expression system: Escherichia coli
UniProt:
  • Canonical: P22629 (Residues: 38-163; Coverage: 79%)
Sequence domains: Avidin family
Strep-tag II peptide Chain: P
Molecule details ›
Chain: P
Length: 12 amino acids
Theoretical weight: 1.34 KDa
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

No bound ligands
1 modified residue:
Ligand BE2 1 x BE2

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: BESSY BEAMLINE 14.2
Spacegroup: I4122
Unit cell:
a: 57.16Å b: 57.16Å c: 175.08Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.202 0.2 0.24
Expression systems:
  • Escherichia coli
  • Not provided

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Citations

4 citation in other articles

Sactipeptide Engineering by Probing the Substrate Tolerance of a Thioether-Bond-Forming Sactisynthase.
Ali et al. (2022)
3 more

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