› 6qwg

X-ray diffraction
1.9Å resolution

Serial Femtosecond Crystallography Structure of Cu Nitrite Reductase from Achromobacter cycloclastes: Nitrite complex at Room Temperature

Released:
DOI: 10.2210/pdb6qwg/pdb
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PDB entry 6qwg coloured by chain, front view.
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Source organism: Achromobacter cycloclastes
Primary publication:
High-throughput structures of protein-ligand complexes at room temperature using serial femtosecond crystallography.
Moreno-Chicano T, Ebrahim A, Axford D, Appleby MV, Beale JH, Chaplin AK, Duyvesteyn HME, Ghiladi RA, Owada S, Sherrell DA, Strange RW, Sugimoto H, Tono K, Worrall JAR, Owen RL, Hough MA
OpenAccess logo IUCrJ 6 1074-1085 (2019)
PMID: 31709063

Function and Biology Details

Reaction catalysed: 
Nitric oxide + H(2)O + ferricytochrome c = nitrite + ferrocytochrome c + 2 H(+)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-150327 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Copper-containing nitrite reductase Chain: A
Molecule details ›
Chain: A
Length: 333 amino acids
Theoretical weight: 36.52 KDa
Source organism: Achromobacter cycloclastes
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P25006 (Residues: 46-378; Coverage: 98%)
Gene name: nirK
Sequence domains:

Ligands and Environments

2 bound ligands:
Ligand NO2 1 x NO2
Ligand CU 2 x CU
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: SACLA BEAMLINE BL2
Spacegroup: P213
Unit cell:
a: 97.6Å b: 97.6Å c: 97.6Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.138 0.137 0.172
Expression system: Escherichia coli BL21(DE3)

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Citations

3 review citations

Best practices for time-resolved serial synchrotron crystallography.
Schulz et al. (2022)
2 more

1 mention without citation

High-throughput structures of protein-ligand complexes at room temperature using serial femtosecond crystallography.
Moreno-Chicano et al. (2019)

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