› 6qwn

X-ray diffraction
3.89Å resolution

Protein peptide complex

Released:
DOI: 10.2210/pdb6qwn/pdb
PDB entry 6qwn coloured by chain, front view.
PDB entry 6qwn coloured by chain, side view.
PDB entry 6qwn coloured by chain, top view.
Source organism: Arabidopsis thaliana
Primary publication:
Structural basis for recognition of RALF peptides by LRX proteins during pollen tube growth.
Moussu S, Broyart C, Santos-Fernandez G, Augustin S, Wehrle S, Grossniklaus U, Santiago J
OpenAccess logo Proc Natl Acad Sci U S A 117 7494-7503 (2020)
PMID: 32165538

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-190389 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (7 distinct):
Pollen-specific leucine-rich repeat extensin-like protein 1 Chains: A, B, C, D, E
Molecule details ›
Chains: A, B, C, D, E
Length: 379 amino acids
Theoretical weight: 41.77 KDa
Source organism: Arabidopsis thaliana
Expression system: Trichoplusia ni
UniProt:
  • Canonical: Q9LJ64 (Residues: 31-400; Coverage: 40%)
Gene names: At3g19020, K13E13.23, PEX1
Sequence domains:
Protein RALF-like 4 Chains: F, G, H, I, J
Molecule details ›
Chains: F, G, H, I, J
Length: 56 amino acids
Theoretical weight: 6.82 KDa
Source organism: Arabidopsis thaliana
Expression system: Trichoplusia ni
UniProt:
  • Canonical: Q9FZA0 (Residues: 54-107; Coverage: 62%)
Gene names: At1g28270, F3H9.8, RALFL4
Sequence domains: Rapid ALkalinization Factor (RALF)

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG, BMA, MAN
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, BMA
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, BMA
Carbohydrate polymer
1 bound ligand:
Ligand NAG 3 x NAG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06DA
Spacegroup: C2
Unit cell:
a: 205.841Å b: 114.091Å c: 146.977Å
α: 90° β: 116.25° γ: 90°
R-values:
R R work R free
0.284 0.282 0.329
Expression system: Trichoplusia ni

Quick links

Citations

14 review citations

RALF-FERONIA Signaling: Linking Plant Immune Response with Cell Growth.
Zhang et al. (2020)
13 more

2 mentions without citation

Structural basis for recognition of RALF peptides by LRX proteins during pollen tube growth.
Moussu et al. (2020)
1 more