› 6qwu

X-ray diffraction
1.4Å resolution

4'-phosphopantetheinyl transferase PptAb from Mycobacterium abscessus at pH 5.5 with Mn2+ and CoA.

Released:
DOI: 10.2210/pdb6qwu/pdb
PDB entry 6qwu coloured by chain, front view.
PDB entry 6qwu coloured by chain, side view.
PDB entry 6qwu coloured by chain, top view.
Source organism: Mycobacteroides abscessus ATCC 19977
Primary publication:
Conformational flexibility of coenzyme A and its impact on the post-translational modification of acyl carrier proteins by 4'-phosphopantetheinyl transferases.
Nguyen MC, Saurel O, Carivenc C, Gavalda S, Saitta S, Tran MP, Milon A, Chalut C, Guilhot C, Mourey L, Pedelacq JD
FEBS J 287 4729-4746 (2020)
PMID: 32128972

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-109340 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
4'-phosphopantetheinyl transferase Chain: A
Molecule details ›
Chain: A
Length: 232 amino acids
Theoretical weight: 25.43 KDa
Source organism: Mycobacteroides abscessus ATCC 19977
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: B1MD73 (Residues: 1-219; Coverage: 100%)
Gene name: MAB_3117c
Sequence domains:

Ligands and Environments

4 bound ligands:
Ligand COA 1 x COA
Ligand MN 2 x MN
Ligand NA 1 x NA
Ligand PEG 1 x PEG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID30B
Spacegroup: P212121
Unit cell:
a: 55.652Å b: 61.918Å c: 66.699Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.165 0.164 0.184
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

1 review citation

Protein-protein interface analysis of the non-ribosomal peptide synthetase peptidyl carrier protein and enzymatic domains.
Corpuz et al. (2022)
1 other article

1 mention without citation

Phosphopantetheinyl transferase binding and inhibition by amidino-urea and hydroxypyrimidinethione compounds.
Carivenc et al. (2021)