› 6qxm

Electron Microscopy
4.1Å resolution

Cryo-EM structure of T7 bacteriophage portal protein, 12mer, open valve

Released:
DOI: 10.2210/pdb6qxm/pdb
PDB entry 6qxm coloured by chain, front view.
PDB entry 6qxm coloured by chain, side view.
PDB entry 6qxm coloured by chain, top view.
Source organism: Escherichia phage T7
Primary publication:
Structures of T7 bacteriophage portal and tail suggest a viral DNA retention and ejection mechanism.
Cuervo A, Fàbrega-Ferrer M, Machón C, Conesa JJ, Fernández FJ, Pérez-Luque R, Pérez-Ruiz M, Pous J, Vega MC, Carrascosa JL, Coll M
OpenAccess logo Nat Commun 10 3746 (2019)
PMID: 31431626
Related structures: EMD-4669

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dodecamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-137237 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Portal protein Chains: A, B, C, D, E, F, G, H, I, J, K, L
Molecule details ›
Chains: A, B, C, D, E, F, G, H, I, J, K, L
Length: 547 amino acids
Theoretical weight: 60.46 KDa
Source organism: Escherichia phage T7
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P03728 (Residues: 1-536; Coverage: 100%)
Gene name: 8
Sequence domains: Bacteriophage head to tail connecting protein

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 4.1Å
Relevant EMDB volumes: EMD-4669
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

5 review citations

Major tail proteins of bacteriophages of the order Caudovirales.
Zinke et al. (2022)
4 more