6qxt

Electron Microscopy
8.9Å resolution

Cas1-Cas2-Csn2-DNA dimer complex from the Type II-A CRISPR-Cas system

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero 54-mer (preferred)
PDBe Complex ID:
PDB-CPX-120515 (preferred)
Entry contents:
3 distinct polypeptide molecules
2 distinct DNA molecules
Macromolecules (5 distinct):
CRISPR-associated protein Csn2 Chains: A, B, C, D, E, F, G, H, a, b, c, d, e, f, g, h
Molecule details ›
Chains: A, B, C, D, E, F, G, H, a, b, c, d, e, f, g, h
Length: 219 amino acids
Theoretical weight: 25.53 KDa
Source organism: Streptococcus thermophilus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: G3ECR4 (Residues: 1-219; Coverage: 100%)
Gene name: csn2
Sequence domains: CRISPR-associated protein (Cas_Csn2)
CRISPR-associated endonuclease Cas1 Chains: I, J, L, M, O, P, R, S, U, V, Y, Z, i, j, l, m, o, p, r, s, u, v, y, z
Molecule details ›
Chains: I, J, L, M, O, P, R, S, U, V, Y, Z, i, j, l, m, o, p, r, s, u, v, y, z
Length: 302 amino acids
Theoretical weight: 35.36 KDa
Source organism: Streptococcus thermophilus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: G3ECR2 (Residues: 1-289; Coverage: 100%)
Gene name: cas1
Sequence domains: CRISPR associated protein Cas1
CRISPR-associated endoribonuclease Cas2 Chains: K, N, Q, T, W, X, k, n, q, t, w, x
DNA (64-MER) Chain: 1
DNA (64-MER) Chain: 2

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 8.9Å
Relevant EMDB volumes: EMD-4670
Expression system: Escherichia coli BL21(DE3)