Sample information
Author description:
Cas1-Cas2-Csn2-DNA dimer complex from the Type II-A CRISPR-Cas system
Source organism:
Expression system:
Escherichia coli BL21(DE3)
Validation information
Experimental information
Specimen preparation
| Buffer name | pH | Details |
|---|---|---|
| - | 7.5 | Buffer solution was filtered |
Vitrification
| Cryogen | Temperature | Instrument | Humidity | Details |
|---|---|---|---|---|
| - | - | FEI VITROBOT MARK IV | 90 | 15 s wait time, 1 s blot time. |
Refinement statistics
Structure solution method:
Electron Microscopy
Resolution:
8.9 Å
