6r0p

X-ray diffraction
1.6Å resolution

Getah virus macro domain in complex with ADPr in double open conformation

Released:

Function and Biology Details

Reactions catalysed:
ATP + RNA(n) = diphosphate + RNA(n+1)
Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1)
ADP-D-ribose 1''-phosphate + H(2)O = ADP-D-ribose + phosphate
NTP + H(2)O = NDP + phosphate
A 5'-triphospho-[mRNA] + H(2)O = a 5'-diphospho-[mRNA] + phosphate
ATP + H(2)O = ADP + phosphate
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
PDBe Complex ID:
PDB-CPX-178524 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Non-structural protein 3 Chains: A, B
Molecule details ›
Chains: A, B
Length: 168 amino acids
Theoretical weight: 18.12 KDa
Source organism: Getah virus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q5Y389 (Residues: 1333-1492; Coverage: 7%)
Sequence domains: Macro domain

Ligands and Environments

3 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE MASSIF-3
Spacegroup: P212121
Unit cell:
a: 46.7Å b: 71.451Å c: 99.503Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.171 0.169 0.194
Expression system: Escherichia coli BL21(DE3)