6r84

Electron Microscopy
3.6Å resolution

Yeast Vms1 (Q295L)-60S ribosomal subunit complex (pre-state with Arb1)

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero 50-mer (preferred)
PDBe Complex ID:
PDB-CPX-135762 (preferred)
Entry contents:
46 distinct polypeptide molecules
4 distinct RNA molecules
Macromolecules (50 distinct):
ABC transporter ATP-binding protein ARB1 Chain: A
Molecule details ›
Chain: A
Length: 519 amino acids
Theoretical weight: 58.6 KDa
Source organism: Saccharomyces cerevisiae
UniProt:
  • Canonical: P40024 (Residues: 85-603; Coverage: 85%)
Gene names: ARB1, YER036C
Sequence domains:
tRNA endonuclease VMS1 Chain: R
Molecule details ›
Chain: R
Length: 433 amino acids
Theoretical weight: 48.65 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Saccharomyces cerevisiae
UniProt:
  • Canonical: Q04311 (Residues: 13-186, 188-529; Coverage: 66%)
Gene names: VMS1, YDR049W
Sequence domains: Bacteroidetes VLRF1 release factor
Eukaryotic translation initiation factor 6 Chain: X
Large ribosomal subunit protein eL34A Chain: i
Large ribosomal subunit protein uL30A Chain: J
Large ribosomal subunit protein uL29A Chain: j
Large ribosomal subunit protein eL8A Chain: K
Large ribosomal subunit protein eL36A Chain: k
Large ribosomal subunit protein uL6A Chain: 7
Large ribosomal subunit protein eL37A Chain: l
Large ribosomal subunit protein uL5B Chain: M
Large ribosomal subunit protein eL38 Chain: m
Large ribosomal subunit protein eL13A Chain: N
Large ribosomal subunit protein eL39 Chain: n
Large ribosomal subunit protein eL14A Chain: O
Large ribosomal subunit protein eL40A Chain: o
Large ribosomal subunit protein eL15A Chain: p
Large ribosomal subunit protein uL13A Chain: Q
Large ribosomal subunit protein uL22A Chain: 5
Large ribosomal subunit protein eL18A Chain: S
Large ribosomal subunit protein uL16 Chain: s
Large ribosomal subunit protein eL19A Chain: T
Large ribosomal subunit protein eL20A Chain: U
Large ribosomal subunit protein eL21A Chain: V
Large ribosomal subunit protein eL22A Chain: W
Large ribosomal subunit protein uL11A Chain: P
Large ribosomal subunit protein uL10 Chain: r
Large ribosomal subunit protein uL14A Chain: x
Large ribosomal subunit protein eL24A Chain: Y
Large ribosomal subunit protein uL23 Chain: Z
Large ribosomal subunit protein uL24A Chain: a
Large ribosomal subunit protein eL27A Chain: b
Large ribosomal subunit protein eL42A Chain: C
Large ribosomal subunit protein uL15 Chain: c
Large ribosomal subunit protein eL43A Chain: D
Large ribosomal subunit protein eL29 Chain: d
Large ribosomal subunit protein uL2A Chain: E
Large ribosomal subunit protein eL30 Chain: e
Large ribosomal subunit protein uL3 Chain: F
Large ribosomal subunit protein eL31A Chain: f
Large ribosomal subunit protein uL4A Chain: G
Large ribosomal subunit protein eL32 Chain: g
Large ribosomal subunit protein uL18 Chain: H
Large ribosomal subunit protein eL33A Chain: h
Large ribosomal subunit protein eL6A Chain: I
Large ribosomal subunit protein uL1A Chain: L
5S ribosomal RNA Chain: 3
5.8S ribosomal RNA Chain: 4
tRNA-Ala Chain: B
25S ribosomal RNA Chain: 1

Ligands and Environments

1 bound ligand:
1 modified residue:

Experiments and Validation Details

Entry percentile scores
Resolution: 3.6Å
Relevant EMDB volumes: EMD-4751
Expression system: Saccharomyces cerevisiae