› 6rak

Electron Microscopy
3.3Å resolution

Heterodimeric ABC exporter TmrAB in vanadate trapped outward-facing occluded conformation

Released:
DOI: 10.2210/pdb6rak/pdb
PDB entry 6rak coloured by chain, front view.
PDB entry 6rak coloured by chain, side view.
PDB entry 6rak coloured by chain, top view.
Source organisms:
Primary publication:
Conformation space of a heterodimeric ABC exporter under turnover conditions.
Hofmann S, Januliene D, Mehdipour AR, Thomas C, Stefan E, Brüchert S, Kuhn BT, Geertsma ER, Hummer G, Tampé R, Moeller A
OpenAccess logo Nature 571 580-583 (2019)
PMID: 31316210
Related structures: EMD-4778

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-280241 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Multidrug resistance ABC transporter ATP-binding and permease protein Chain: A
Molecule details ›
Chain: A
Length: 623 amino acids
Theoretical weight: 70.66 KDa
Source organism: Thermus thermophilus
Expression system: Escherichia coli
Multidrug resistance ABC transporter ATP-binding and permease protein Chain: B
Molecule details ›
Chain: B
Length: 578 amino acids
Theoretical weight: 64.63 KDa
Source organism: Thermus thermophilus
Expression system: Escherichia coli
Nanobody Nb9F10 Chain: C

Ligands and Environments

3 bound ligands:
Ligand AOV 1 x AOV
Ligand MG 2 x MG
Ligand ATP 1 x ATP
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.3Å
Relevant EMDB volumes: EMD-4778
Expression system: Escherichia coli

Quick links

Citations

24 review citations

Structure, Assembly, and Function of Tripartite Efflux and Type 1 Secretion Systems in Gram-Negative Bacteria.
Alav et al. (2021)
23 more

8 mentions without citation

Structural and functional diversity calls for a new classification of ABC transporters.
Thomas et al. (2020)
7 more