6rao

Electron Microscopy
3.1Å resolution

Cryo-EM structure of the anti-feeding prophage (AFP) baseplate, 6-fold symmetrised

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero 60-mer (preferred)
PDBe Complex ID:
PDB-CPX-179733 (preferred)
Entry contents:
9 distinct polypeptide molecules
Macromolecules (9 distinct):
Afp1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 149 amino acids
Theoretical weight: 16.45 KDa
Source organism: Serratia entomophila
Expression system: Escherichia coli
UniProt:
  • Canonical: Q6HAD8 (Residues: 1-149; Coverage: 100%)
Gene name: afp1
Sequence domains: T4-like virus tail tube protein gp19
Afp2 Chain: C
Molecule details ›
Chain: C
Length: 354 amino acids
Theoretical weight: 38.78 KDa
Source organism: Serratia entomophila
Expression system: Escherichia coli
UniProt:
  • Canonical: Q6HAD7 (Residues: 1-354; Coverage: 100%)
Gene name: afp2
Sequence domains:
Afp3 Chain: D
Molecule details ›
Chain: D
Length: 451 amino acids
Theoretical weight: 48.78 KDa
Source organism: Serratia entomophila
Expression system: Escherichia coli
UniProt:
  • Canonical: Q6HAD6 (Residues: 1-451; Coverage: 100%)
Gene name: afp3
Sequence domains:
Phage tail protein Chain: F
Molecule details ›
Chain: F
Length: 149 amino acids
Theoretical weight: 17.03 KDa
Source organism: Serratia entomophila
Expression system: Escherichia coli
UniProt:
  • Canonical: Q6HAD4 (Residues: 1-149; Coverage: 100%)
Gene name: afp5
Sequence domains: T4-like virus tail tube protein gp19
IraD/Gp25-like domain-containing protein Chain: H
Molecule details ›
Chain: H
Length: 140 amino acids
Theoretical weight: 15.69 KDa
Source organism: Serratia entomophila
Expression system: Escherichia coli
UniProt:
  • Canonical: Q6HAD0 (Residues: 1-140; Coverage: 100%)
Gene name: afp9
Sequence domains: Baseplate wedge protein gp25
Afp4 Chain: E
Molecule details ›
Chain: E
Length: 417 amino acids
Theoretical weight: 45.57 KDa
Source organism: Serratia entomophila
Expression system: Escherichia coli
UniProt:
  • Canonical: Q6HAD5 (Residues: 1-417; Coverage: 100%)
Gene name: afp4
Contractile injection system tube protein N-terminal domain-containing protein Chain: G
Molecule details ›
Chain: G
Length: 229 amino acids
Theoretical weight: 25.26 KDa
Source organism: Serratia entomophila
Expression system: Escherichia coli
UniProt:
  • Canonical: Q6HAD2 (Residues: 1-229; Coverage: 100%)
Gene name: afp7
Sequence domains: Contractile injection system tube protein
Afp12 Chain: I
Molecule details ›
Chain: I
Length: 607 amino acids
Theoretical weight: 67.33 KDa
Source organism: Serratia entomophila
Expression system: Escherichia coli
UniProt:
  • Canonical: Q6HAC8 (Residues: 1-607; Coverage: 100%)
Gene name: afp11
Afp12 Chain: J
Molecule details ›
Chain: J
Length: 963 amino acids
Theoretical weight: 106.91 KDa
Source organism: Serratia entomophila
Expression system: Escherichia coli
UniProt:
  • Canonical: Q6HAC7 (Residues: 1-963; Coverage: 100%)
Gene name: afp12

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.1Å
Relevant EMDB volumes: EMD-4782
Expression system: Escherichia coli