› 6ray

Electron Microscopy
4.28Å resolution

D. melanogaster CMG-DNA, State 2A

Released:
DOI: 10.2210/pdb6ray/pdb
PDB entry 6ray coloured by chain, front view.
PDB entry 6ray coloured by chain, side view.
PDB entry 6ray coloured by chain, top view.
Source organism: Drosophila melanogaster
Primary publication:
Molecular Basis for ATP-Hydrolysis-Driven DNA Translocation by the CMG Helicase of the Eukaryotic Replisome.
Eickhoff P, Kose HB, Martino F, Petojevic T, Abid Ali F, Locke J, Tamberg N, Nans A, Berger JM, Botchan MR, Yardimci H, Costa A
OpenAccess logo Cell Rep 28 2673-2688.e8 (2019)
PMID: 31484077
Related structures: EMD-4787

Function and Biology Details

Reaction catalysed: 
ATP + H(2)O = ADP + phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dodecamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-120538 (preferred)
Entry contents:
11 distinct polypeptide molecules
1 distinct DNA molecule
Macromolecules (12 distinct):
DNA replication licensing factor Mcm3 Chain: 3
Molecule details ›
Chain: 3
Length: 819 amino acids
Theoretical weight: 91.05 KDa
Source organism: Drosophila melanogaster
Expression system: Trichoplusia ni
UniProt:
  • Canonical: Q9XYU1 (Residues: 1-819; Coverage: 100%)
Gene names: CG4206, Mcm3, Mcm3-RA
Sequence domains:
DNA replication licensing factor MCM4 Chain: 4
Molecule details ›
Chain: 4
Length: 866 amino acids
Theoretical weight: 96.74 KDa
Source organism: Drosophila melanogaster
Expression system: Trichoplusia ni
UniProt:
  • Canonical: Q26454 (Residues: 1-866; Coverage: 100%)
Gene names: CG1616, dpa
Sequence domains:
DNA replication licensing factor Mcm7 Chain: 7
DNA replication licensing factor Mcm6 Chain: 6
DNA replication licensing factor Mcm5 Chain: 5
LD35753p Chain: A
DNA replication complex GINS protein PSF1 Chain: H
Probable DNA replication complex GINS protein PSF2 Chain: L
DNA replication complex GINS protein PSF3 Chain: M
DNA replication complex GINS protein SLD5 Chain: N
DNA replication licensing factor Mcm2 Chain: 2
DNA (5'-D(P*AP*TP*TP*TP*TP*TP*TP*TP*TP*TP*TP*TP*T)-3') Chain: X

Ligands and Environments

2 bound ligands:
Ligand ATP 4 x ATP
Ligand ADP 2 x ADP
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 4.28Å
Relevant EMDB volumes: EMD-4787
Expression system: Trichoplusia ni

Quick links

Citations

17 review citations

AAA+ ATPases: structural insertions under the magnifying glass.
Jessop et al. (2021)
16 more

1 mention without citation

Molecular Basis for ATP-Hydrolysis-Driven DNA Translocation by the CMG Helicase of the Eukaryotic Replisome.
Eickhoff et al. (2019)