› 6rb7

X-ray diffraction
1.6Å resolution

Function and Biology Details

Reaction catalysed: 
Aceneneuramate = N-acetyl-D-mannosamine + pyruvate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-108235 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
N-acetylneuraminate lyase Chains: A, B, E, F
Molecule details ›
Chains: A, B, E, F
Length: 336 amino acids
Theoretical weight: 37.1 KDa
Source organism: [Ruminococcus] gnavus ATCC 29149
Expression system: Escherichia coli
UniProt:
  • Canonical: A7B555 (Residues: 11-315; Coverage: 97%)
Gene names: RUMGNA_02692, nanA
Sequence domains: Dihydrodipicolinate synthetase family

Ligands and Environments

5 bound ligands:
Ligand TRS 4 x TRS
Ligand GOL 6 x GOL
Ligand PEG 3 x PEG
Ligand BCN 1 x BCN
Ligand MG 4 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04
Spacegroup: P21
Unit cell:
a: 73.181Å b: 121.39Å c: 86.98Å
α: 90° β: 109.49° γ: 90°
R-values:
R R work R free
0.128 0.128 0.151
Expression system: Escherichia coli

Quick links

Citations

15 review citations

The microbiome in inflammatory bowel diseases: from pathogenesis to therapy.
Liu et al. (2021)
14 more

3 mentions without citation

Slimy partners: the mucus barrier and gut microbiome in ulcerative colitis.
Fang et al. (2021)
2 more