› 6rc8

Electron Microscopy
3.8Å resolution

Cryo-EM structure of the anti-feeding prophage (AFP) helical sheath in contracted state

Released:
DOI: 10.2210/pdb6rc8/pdb
PDB entry 6rc8 coloured by chain, front view.
PDB entry 6rc8 coloured by chain, side view.
PDB entry 6rc8 coloured by chain, top view.
Source organism: Serratia entomophila
Primary publication:
Atomic structures of an entire contractile injection system in both the extended and contracted states.
Desfosses A, Venugopal H, Joshi T, Felix J, Jessop M, Jeong H, Hyun J, Heymann JB, Hurst MRH, Gutsche I, Mitra AK
OpenAccess logo Nat Microbiol 4 1885-1894 (2019)
PMID: 31384001
Related structures: EMD-4803

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero 48-mer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-179737 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Afp2 Chain: A
Molecule details ›
Chain: A
Length: 354 amino acids
Theoretical weight: 38.78 KDa
Source organism: Serratia entomophila
Expression system: Escherichia coli
UniProt:
  • Canonical: Q6HAD7 (Residues: 1-354; Coverage: 100%)
Gene name: afp2
Sequence domains:
Afp3 Chain: B
Molecule details ›
Chain: B
Length: 451 amino acids
Theoretical weight: 48.78 KDa
Source organism: Serratia entomophila
Expression system: Escherichia coli
UniProt:
  • Canonical: Q6HAD6 (Residues: 1-451; Coverage: 100%)
Gene name: afp3
Sequence domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.8Å
Relevant EMDB volumes: EMD-4803
Expression system: Escherichia coli

Quick links

Citations

3 review citations

Major tail proteins of bacteriophages of the order Caudovirales.
Zinke et al. (2022)
2 more

1 mention without citation

Evolution of Phage Tail Sheath Protein.
Evseev et al. (2022)