› 6rev

Electron Microscopy
3.8Å resolution

Cryo-EM structure of the N-terminal DC repeat (NDC) of human doublecortin (DCX) bound to 13-protofilament GDP-microtubule

Released:
DOI: 10.2210/pdb6rev/pdb
PDB entry 6rev coloured by chain, front view.
PDB entry 6rev coloured by chain, side view.
PDB entry 6rev coloured by chain, top view.
Source organisms:
Primary publication:
Pseudo-repeats in doublecortin make distinct mechanistic contributions to microtubule regulation.
Manka SW, Moores CA
OpenAccess logo EMBO Rep 21 e51534 (2020)
PMID: 33051979
Related structures: EMD-4858

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
PDBe Complex ID:
PDB-CPX-129018 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Neuronal migration protein doublecortin Chain: N
Molecule details ›
Chain: N
Length: 99 amino acids
Theoretical weight: 11.44 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: O43602 (Residues: 44-142; Coverage: 27%)
Gene names: DBCN, DCX, LISX
Sequence domains: Doublecortin
Tubulin beta-2B chain Chains: B, b
Molecule details ›
Chains: B, b
Length: 429 amino acids
Theoretical weight: 48.11 KDa
Source organism: Bos taurus
UniProt:
  • Canonical: Q6B856 (Residues: 1-429; Coverage: 96%)
Gene name: TUBB2B
Sequence domains:
Tubulin alpha-1B chain Chains: A, a

Ligands and Environments

3 bound ligands:
Ligand GDP 2 x GDP
Ligand GTP 2 x GTP
Ligand MG 2 x MG
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this EM entry.
Resolution: 3.8Å
Relevant EMDB volumes: EMD-4858
Expression system: Escherichia coli

Quick links

Citations

4 review citations

Mechanisms of microtubule organization in differentiated animal cells.
Akhmanova et al. (2022)
3 more

5 mentions without citation

Structure-Guided Prediction of the Functional Impact of DCLK1 Mutations on Tumorigenesis.
Carli et al. (2023)
4 more