› 6rje

X-ray diffraction
2.5Å resolution

Lysostaphin SH3b P4-G5 complex, homesource dataset

Released:
DOI: 10.2210/pdb6rje/pdb
PDB entry 6rje coloured by chain, front view.
PDB entry 6rje coloured by chain, side view.
PDB entry 6rje coloured by chain, top view.
Source organism: Staphylococcus simulans
Primary publication:
Two-site recognition of Staphylococcus aureus peptidoglycan by lysostaphin SH3b.
Gonzalez-Delgado LS, Walters-Morgan H, Salamaga B, Robertson AJ, Hounslow AM, Jagielska E, Sabała I, Williamson MP, Lovering AL, Mesnage S
OpenAccess logo Nat Chem Biol 16 24-30 (2020)
PMID: 31686030

Function and Biology Details

Reaction catalysed: 
Hydrolysis of the -Gly-|-Gly- bond in the pentaglycine inter-peptide link joining staphylococcal cell wall peptidoglycans.
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-145336 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Lysostaphin Chain: A
Molecule details ›
Chain: A
Length: 100 amino acids
Theoretical weight: 11.24 KDa
Source organism: Staphylococcus simulans
Expression system: Escherichia coli
UniProt:
  • Canonical: P10547 (Residues: 401-493; Coverage: 20%)
Gene name: lss
Sequence domains: Bacterial SH3 domain

Ligands and Environments

1 bound ligand:
Ligand K5T 1 x K5T
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007
Spacegroup: P41212
Unit cell:
a: 47.19Å b: 47.19Å c: 123.06Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.256 0.254 0.29
Expression system: Escherichia coli

Quick links

Citations

6 review citations

The microbiome-shaping roles of bacteriocins.
Heilbronner et al. (2021)
5 more

2 mentions without citation

Two-site recognition of Staphylococcus aureus peptidoglycan by lysostaphin SH3b.
Gonzalez-Delgado et al. (2020)
1 more