› 6rkx

X-ray diffraction
2.09Å resolution

The crystal structure of AbnE, an arabino-oligosaccharide binding protein, in complex with arabinopentaose

Released:
DOI: 10.2210/pdb6rkx/pdb
PDB entry 6rkx coloured by chain, front view.
PDB entry 6rkx coloured by chain, side view.
PDB entry 6rkx coloured by chain, top view.
Source organism: Geobacillus stearothermophilus
Primary publication:
Carbohydrate-Binding Capability and Functional Conformational Changes of AbnE, an Arabino-oligosaccharide Binding Protein.
Lansky S, Salama R, Shulami S, Lavid N, Sen S, Schapiro I, Shoham Y, Shoham G
J Mol Biol 432 2099-2120 (2020)
PMID: 32067952

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-109834 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Arabino-oligosaccharids-binding protein Chain: A
Molecule details ›
Chain: A
Length: 427 amino acids
Theoretical weight: 48.69 KDa
Source organism: Geobacillus stearothermophilus
Expression system: Escherichia coli
UniProt:
  • Canonical: B3EYM9 (Residues: 34-454; Coverage: 98%)
Gene name: abnE
Sequence domains: Bacterial extracellular solute-binding protein

Ligands and Environments

Carbohydrate polymer : NEW Components: AHR
Carbohydrate polymer
2 bound ligands:
Ligand GOL 3 x GOL
Ligand CA 1 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE BM14
Spacegroup: P212121
Unit cell:
a: 39.151Å b: 95.113Å c: 119.449Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.186 0.182 0.246
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Biophysical Approaches for the Characterization of Protein-Metabolite Interactions.
Thalhammer et al. (2023)
1 more