› 6rm2

X-ray diffraction
2.5Å resolution

Deoxyguanylosuccinate synthase (DgsS) structure with ATP, IMP, Magnesium

Released:
DOI: 10.2210/pdb6rm2/pdb
PDB entry 6rm2 coloured by chain, front view.
PDB entry 6rm2 coloured by chain, side view.
PDB entry 6rm2 coloured by chain, top view.
Source organism: Vibrio phage phiVC8
Entry authors: Sleiman D, Loc'h J, Haouz A, Kaminski PA

Function and Biology Details

Reaction catalysed: 
ATP + dGMP + L-aspartate = ADP + phosphate + 2-amino-2'-deoxy-N(6)-((2S)-succino)adenylate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
homo tetramer
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-124326 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
N6-succino-2-amino-2'-deoxyadenylate synthase Chains: A, B
Molecule details ›
Chains: A, B
Length: 363 amino acids
Theoretical weight: 40.39 KDa
Source organism: Vibrio phage phiVC8
Expression system: Escherichia coli
UniProt:
  • Canonical: G3FFN6 (Residues: 1-343; Coverage: 100%)
Gene names: phiVC8_p27, purZ
Sequence domains: Adenylosuccinate synthetase

Ligands and Environments

4 bound ligands:
Ligand ATP 2 x ATP
Ligand MG 2 x MG
Ligand IMP 2 x IMP
Ligand SO4 2 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SOLEIL BEAMLINE PROXIMA 1
Spacegroup: P41212
Unit cell:
a: 90.82Å b: 90.82Å c: 192.17Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.18 0.177 0.231
Expression system: Escherichia coli

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