› 6rnb

X-ray diffraction
1.7Å resolution

Liquid Application Method for time-resolved Analyses (LAMA) by serial synchrotron crystallography, Lysozyme with GlcNAc3 50ms diffusion time

Released:
DOI: 10.2210/pdb6rnb/pdb
PDB entry 6rnb coloured by chain, front view.
PDB entry 6rnb coloured by chain, side view.
PDB entry 6rnb coloured by chain, top view.
Source organism: Gallus gallus
Primary publication:
Liquid application method for time-resolved analyses by serial synchrotron crystallography.
Mehrabi P, Schulz EC, Agthe M, Horrell S, Bourenkov G, von Stetten D, Leimkohl JP, Schikora H, Schneider TR, Pearson AR, Tellkamp F, Miller RJD
Nat Methods 16 979-982 (2019)
PMID: 31527838

Function and Biology Details

Reaction catalysed: 
Hydrolysis of (1->4)-beta-linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in a peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrins
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-132831 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Lysozyme C Chain: A
Molecule details ›
Chain: A
Length: 129 amino acids
Theoretical weight: 14.33 KDa
Source organism: Gallus gallus
UniProt:
  • Canonical: P00698 (Residues: 19-147; Coverage: 100%)
Gene name: LYZ
Sequence domains: C-type lysozyme/alpha-lactalbumin family

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
2 bound ligands:
Ligand CL 2 x CL
Ligand NA 1 x NA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PETRA III, EMBL c/o DESY BEAMLINE P14 (MX2)
Spacegroup: P43212
Unit cell:
a: 78.7Å b: 78.7Å c: 37.9Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.17 0.169 0.186

Quick links

Citations

9 review citations

Best practices for time-resolved serial synchrotron crystallography.
Schulz et al. (2022)
8 more