› 6rnx

X-ray diffraction
2.84Å resolution

Crystal structure of the essential repressor DdrO from radiation-resistant Deinococcus bacteria (Deinococcus deserti)

Released:
DOI: 10.2210/pdb6rnx/pdb
PDB entry 6rnx coloured by chain, front view.
PDB entry 6rnx coloured by chain, side view.
PDB entry 6rnx coloured by chain, top view.
Source organism: Deinococcus deserti VCD115
Primary publication:
Crystal structure of the transcriptional repressor DdrO: insight into the metalloprotease/repressor-controlled radiation response in Deinococcus.
de Groot A, Siponen MI, Magerand R, Eugénie N, Martin-Arevalillo R, Doloy J, Lemaire D, Brandelet G, Parcy F, Dumas R, Roche P, Servant P, Confalonieri F, Arnoux P, Pignol D, Blanchard L
OpenAccess logo Nucleic Acids Res 47 11403-11417 (2019)
PMID: 31598697

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-111079 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
HTH-type transcriptional regulator DdrOC Chains: A, B
Molecule details ›
Chains: A, B
Length: 129 amino acids
Theoretical weight: 14.76 KDa
Source organism: Deinococcus deserti VCD115
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'
UniProt:
  • Canonical: C1CYP4 (Residues: 1-129; Coverage: 100%)
Gene names: Deide_20570, ddrOC
Sequence domains: Helix-turn-helix

Ligands and Environments

1 bound ligand:
Ligand CL 1 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-2
Spacegroup: C2221
Unit cell:
a: 32.902Å b: 130.188Å c: 154.766Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.226 0.222 0.296
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'

Quick links

Citations

2 review citations

Coexistence of SOS-Dependent and SOS-Independent Regulation of DNA Repair Genes in Radiation-Resistant Deinococcus Bacteria.
Blanchard et al. (2021)
1 more

2 mentions without citation

Structural features and functional implications of proteins enabling the robustness of Deinococcus radiodurans.
Chen et al. (2020)
1 more