› 6ru7

X-ray diffraction
2.08Å resolution

Crystal structure of Casein Kinase I delta (CK1d) in complex with double phosphorylated p63 PAD2P peptide

Released:
DOI: 10.2210/pdb6ru7/pdb
PDB entry 6ru7 coloured by chain, front view.
PDB entry 6ru7 coloured by chain, side view.
PDB entry 6ru7 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
p63 uses a switch-like mechanism to set the threshold for induction of apoptosis.
Gebel J, Tuppi M, Chaikuad A, Hötte K, Schröder M, Schulz L, Löhr F, Gutfreund N, Finke F, Henrich E, Mezhyrova J, Lehnert R, Pampaloni F, Hummer G, Stelzer EHK, Knapp S, Dötsch V
Nat Chem Biol 16 1078-1086 (2020)
PMID: 32719556

Function and Biology Details

Reactions catalysed: 
ATP + a protein = ADP + a phosphoprotein
ATP + [tau protein] = ADP + [tau protein] phosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-155797 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Casein kinase I isoform delta Chains: A, B
Molecule details ›
Chains: A, B
Length: 296 amino acids
Theoretical weight: 34.42 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P48730 (Residues: 1-294; Coverage: 71%)
Gene names: CSNK1D, HCKID
Sequence domains: Protein kinase domain
Tumor protein 63 Chains: C, D
Molecule details ›
Chains: C, D
Length: 16 amino acids
Theoretical weight: 1.72 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: Q9H3D4 (Residues: 618-633; Coverage: 2%)
Gene names: KET, P63, P73H, P73L, TP63, TP73L

Ligands and Environments

3 bound ligands:
Ligand ADP 2 x ADP
Ligand EDO 17 x EDO
Ligand NA 1 x NA
1 modified residue:
Ligand SEP 4 x SEP

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Spacegroup: C2
Unit cell:
a: 171.779Å b: 48.673Å c: 87.631Å
α: 90° β: 109.93° γ: 90°
R-values:
R R work R free
0.178 0.175 0.218
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

7 review citations

DNA Damaged Induced Cell Death in Oocytes.
Gebel et al. (2020)
6 more

3 mentions without citation

Kinase domain autophosphorylation rewires the activity and substrate specificity of CK1 enzymes.
Cullati et al. (2022)
2 more