› 6ruf

X-ray diffraction
2Å resolution

Wolinella succinogenes L-asparaginase mutant V23Q,K24T with L-Glu

Released:
DOI: 10.2210/pdb6ruf/pdb
PDB entry 6ruf coloured by chain, front view.
PDB entry 6ruf coloured by chain, side view.
PDB entry 6ruf coloured by chain, top view.
Source organism: Wolinella succinogenes DSM 1740
Primary publication:
Features of the Three Dimensional Structure of the Mutant Form of Wolinella succinogenes L-Asparaginase in Complexes with L-Aspartic and L-Glutamic Acids
Timofeev VI, Zhukhlistova NE, Kuranova IP
Russ. J. Bioorganic Chem. 46 (2020)

Function and Biology Details

Reaction catalysed: 
L-asparagine + H(2)O = L-aspartate + NH(3)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-156164 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
L-asparaginase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 328 amino acids
Theoretical weight: 34.7 KDa
Source organism: Wolinella succinogenes DSM 1740
Expression system: Escherichia coli
UniProt:
  • Canonical: P50286 (Residues: 3-330; Coverage: 99%)
Gene names: WS0660, ansA, ansB
Sequence domains:

Ligands and Environments

1 bound ligand:
Ligand GLU 4 x GLU
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL41XU
Spacegroup: P21
Unit cell:
a: 64.76Å b: 110.29Å c: 89.9Å
α: 90° β: 97.72° γ: 90°
R-values:
R R work R free
0.218 0.215 0.264
Expression system: Escherichia coli

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