› 6rzn

X-ray diffraction
1.91Å resolution

Crystal structure of the N-terminal carbohydrate binding module family 48 and ferulic acid esterase from the multi-enzyme CE1-GH62-GH10

Released:
DOI: 10.2210/pdb6rzn/pdb
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PDB entry 6rzn coloured by chain, front view.
PDB entry 6rzn coloured by chain, side view.
PDB entry 6rzn coloured by chain, top view.
Source organism: uncultured bacterium
Primary publication:
A carbohydrate-binding family 48 module enables feruloyl esterase action on polymeric arabinoxylan.
Holck J, Fredslund F, Møller MS, Brask J, Krogh KBRM, Lange L, Welner DH, Svensson B, Meyer AS, Wilkens C
J Biol Chem 294 17339-17353 (2019)
PMID: 31558605

Function and Biology Details

Reaction catalysed: 
Feruloyl-polysaccharide + H(2)O = ferulate + polysaccharide
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-105483 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glycoside hydrolase family 13 N-terminal domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 386 amino acids
Theoretical weight: 42.79 KDa
Source organism: uncultured bacterium
Expression system: Escherichia coli
UniProt:
  • Canonical: A0A5S8WFA0 (Residues: 1-386; Coverage: 100%)
Sequence domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: ESRF BEAMLINE MASSIF-3
Spacegroup: P212121
Unit cell:
a: 65.65Å b: 99.9Å c: 133.28Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.177 0.176 0.205
Expression system: Escherichia coli

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Citations

8 citation in other articles

Degradation Products of Complex Arabinoxylans by Bacteroides intestinalis Enhance the Host Immune Response.
Yasuma et al. (2021)
7 more

3 mentions without citation

A carbohydrate-binding family 48 module enables feruloyl esterase action on polymeric arabinoxylan.
Holck et al. (2019)
2 more

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