› 6s0h

X-ray diffraction
2.85Å resolution

Structure of IMP-13 metallo-beta-lactamase complexed with hydrolysed doripenem

Released:
DOI: 10.2210/pdb6s0h/pdb
PDB entry 6s0h coloured by chain, front view.
PDB entry 6s0h coloured by chain, side view.
PDB entry 6s0h coloured by chain, top view.
Source organism: Pseudomonas aeruginosa
Primary publication:
Structure and Molecular Recognition Mechanism of IMP-13 Metallo-β-Lactamase.
Softley CA, Zak KM, Bostock MJ, Fino R, Zhou RX, Kolonko M, Mejdi-Nitiu R, Meyer H, Sattler M, Popowicz GM
OpenAccess logo Antimicrob Agents Chemother 64 (2020)
PMID: 32205343

Function and Biology Details

Reaction catalysed: 
A beta-lactam + H(2)O = a substituted beta-amino acid
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-566287 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Metallo-beta-lactamase type 2 Chains: A, B
Molecule details ›
Chains: A, B
Length: 227 amino acids
Theoretical weight: 25.17 KDa
Source organism: Pseudomonas aeruginosa
Expression system: Escherichia coli
UniProt:
  • Canonical: Q7WYA8 (Residues: 21-246; Coverage: 100%)
Gene names: bla-imp13, blaIMP

Ligands and Environments

3 bound ligands:
Ligand ZN 4 x ZN
Ligand EDO 8 x EDO
Ligand KQ8 2 x KQ8
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06DA
Spacegroup: P21
Unit cell:
a: 61.43Å b: 72.318Å c: 62.933Å
α: 90° β: 111.72° γ: 90°
R-values:
R R work R free
0.23 0.228 0.263
Expression system: Escherichia coli

Quick links

Citations

3 citation in other articles

Different Conformations Revealed by NMR Underlie Resistance to Ceftazidime/Avibactam and Susceptibility to Meropenem and Imipenem among D179Y Variants of KPC β-Lactamase.
Taracila et al. (2022)
2 more

2 mentions without citation

Metallo-β-lactamases in the Age of Multidrug Resistance: From Structure and Mechanism to Evolution, Dissemination, and Inhibitor Design.
Bahr et al. (2021)
1 more