6s13

Electron Microscopy
3.58Å resolution

Erythromycin Resistant Staphylococcus aureus 70S ribosome (delta R88 A89 uL22).

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero 52-mer (preferred)
PDBe Complex ID:
PDB-CPX-492626 (preferred)
Entry contents:
49 distinct polypeptide molecules
3 distinct RNA molecules
Macromolecules (52 distinct):
Large ribosomal subunit protein uL2 Chain: C
Molecule details ›
Chain: C
Length: 274 amino acids
Theoretical weight: 29.83 KDa
Source organism: Staphylococcus aureus
UniProt:
  • Canonical: B3VKN3 (Residues: 2-275; Coverage: 99%)
Gene names: rap, rplB
Sequence domains:
Large ribosomal subunit protein uL3 Chain: D
Molecule details ›
Chain: D
Length: 215 amino acids
Theoretical weight: 23.2 KDa
Source organism: Staphylococcus aureus
UniProt:
  • Canonical: W8U3W0 (Residues: 2-216; Coverage: 98%)
Gene names: CD116_01800, ERS140147_00638, NCTC13712_02198, rplC
Sequence domains: Ribosomal protein L3
Large ribosomal subunit protein uL13 Chain: H
Molecule details ›
Chain: H
Length: 145 amino acids
Theoretical weight: 16.36 KDa
Source organism: Staphylococcus aureus
UniProt:
  • Canonical: W8TUE6 (Residues: 1-145; Coverage: 100%)
Gene names: CD116_01960, ERS140147_00605, NCTC13712_02165, rplM
Sequence domains: Ribosomal protein L13
Large ribosomal subunit protein uL14 Chain: I
Molecule details ›
Chain: I
Length: 122 amino acids
Theoretical weight: 13.16 KDa
Source organism: Staphylococcus aureus
UniProt:
  • Canonical: A0A077UUA0 (Residues: 1-122; Coverage: 100%)
Gene names: CD116_01850, ERS140147_00628, NCTC13712_02188, rplN
Sequence domains: Ribosomal protein L14p/L23e
Large ribosomal subunit protein uL15 Chain: J
Molecule details ›
Chain: J
Length: 146 amino acids
Theoretical weight: 15.63 KDa
Source organism: Staphylococcus aureus
UniProt:
  • Canonical: A0A077UGA7 (Residues: 1-146; Coverage: 100%)
Gene names: CD116_01895, ERS140147_00619, NCTC13712_02179, rplO
Sequence domains: Ribosomal proteins 50S-L15, 50S-L18e, 60S-L27A
Large ribosomal subunit protein uL16 Chain: K
Molecule details ›
Chain: K
Length: 137 amino acids
Theoretical weight: 15.6 KDa
Source organism: Staphylococcus aureus
UniProt:
  • Canonical: A0A077V4G0 (Residues: 1-137; Coverage: 95%)
Gene names: CD116_01835, ERS140147_00631, NCTC13712_02191, rplP
Sequence domains: Ribosomal protein L16p/L10e
Large ribosomal subunit protein bL17 Chain: L
Molecule details ›
Chain: L
Length: 120 amino acids
Theoretical weight: 13.58 KDa
Source organism: Staphylococcus aureus
UniProt:
  • Canonical: A0A077UG91 (Residues: 3-122; Coverage: 98%)
Gene names: CD116_01935, ERS140147_00611, NCTC13712_02171, rplQ
Sequence domains: Ribosomal protein L17
Large ribosomal subunit protein bL19 Chain: N
Molecule details ›
Chain: N
Length: 114 amino acids
Theoretical weight: 13.1 KDa
Source organism: Staphylococcus aureus
UniProt:
  • Canonical: A0A077UVB6 (Residues: 2-115; Coverage: 98%)
Gene names: CD116_13000, ERS140147_00145, NCTC13712_01186, rplS
Sequence domains: Ribosomal protein L19
Large ribosomal subunit protein bL20 Chain: O
Molecule details ›
Chain: O
Length: 116 amino acids
Theoretical weight: 13.46 KDa
Source organism: Staphylococcus aureus
UniProt:
  • Canonical: A0A077VMP6 (Residues: 2-117; Coverage: 98%)
Gene names: CD116_08935, ERS140147_00901, NCTC13712_01667, rplT
Sequence domains: Ribosomal protein L20
Large ribosomal subunit protein uL22 Chain: Q
Molecule details ›
Chain: Q
Length: 110 amino acids
Theoretical weight: 12.1 KDa
Source organism: Staphylococcus aureus
UniProt:
  • Canonical: A0A077UKF9 (Residues: 1-112; Coverage: 94%)
Gene names: CD116_01825, ERS140147_00633, NCTC13712_02193, rplV
Sequence domains: Ribosomal protein L22p/L17e
50S ribosomal protein L25 Chain: T
Molecule details ›
Chain: T
Length: 94 amino acids
Theoretical weight: 10.46 KDa
Source organism: Staphylococcus aureus
Large ribosomal subunit protein bL27 Chain: U
Molecule details ›
Chain: U
Length: 82 amino acids
Theoretical weight: 8.91 KDa
Source organism: Staphylococcus aureus
UniProt:
  • Canonical: A0A077ULC5 (Residues: 12-93; Coverage: 87%)
Gene names: CD116_08800, ERS140147_00927, NCTC13712_01641, rpmA
Sequence domains: Ribosomal L27 protein
Large ribosomal subunit protein bL28 Chain: V
Molecule details ›
Chain: V
Length: 58 amino acids
Theoretical weight: 6.55 KDa
Source organism: Staphylococcus aureus
UniProt:
  • Canonical: A0A077URJ8 (Residues: 3-60; Coverage: 94%)
Gene names: CD116_12910, ERS140147_00162, NCTC13712_01169, rpmB
Sequence domains: Ribosomal L28 family
Large ribosomal subunit protein uL29 Chain: W
Molecule details ›
Chain: W
Length: 67 amino acids
Theoretical weight: 7.85 KDa
Source organism: Staphylococcus aureus
UniProt:
  • Canonical: A0A077W1J5 (Residues: 2-68; Coverage: 97%)
Gene names: CD116_01840, ERS140147_00630, NCTC13712_02190, rpmC
Sequence domains: Ribosomal L29 protein
Large ribosomal subunit protein bL31B Chain: Y
Molecule details ›
Chain: Y
Length: 59 amino acids
Theoretical weight: 6.91 KDa
Source organism: Staphylococcus aureus
UniProt:
  • Canonical: A0A380EL30 (Residues: 1-59; Coverage: 47%)
Gene names: NCTC10702_03348, rpmE2
Sequence domains: Ribosomal protein L31
50S ribosomal protein L32 Chain: Z
Molecule details ›
Chain: Z
Length: 48 amino acids
Theoretical weight: 5.57 KDa
Source organism: Staphylococcus aureus
Large ribosomal subunit protein bL33 Chain: 1
Molecule details ›
Chain: 1
Length: 47 amino acids
Theoretical weight: 5.68 KDa
Source organism: Staphylococcus aureus
UniProt:
  • Canonical: A0A077V2P0 (Residues: 2-48; Coverage: 96%)
Gene names: CD116_07290, ERS140147_01724, NCTC13712_01328, rpmG, rpmG2, rpmG2_1
Sequence domains: Ribosomal protein L33
Large ribosomal subunit protein bL34 Chain: 2
Molecule details ›
Chain: 2
Length: 43 amino acids
Theoretical weight: 5.25 KDa
Source organism: Staphylococcus aureus
UniProt:
  • Canonical: W8TQ59 (Residues: 2-44; Coverage: 96%)
Gene names: CD116_00205, ERS140147_01157, NCTC13712_02668, rpmH
Sequence domains: Ribosomal protein L34
Large ribosomal subunit protein bL35 Chain: 3
Molecule details ›
Chain: 3
Length: 64 amino acids
Theoretical weight: 7.46 KDa
Source organism: Staphylococcus aureus
UniProt:
  • Canonical: A0A077UL47 (Residues: 2-65; Coverage: 97%)
Gene names: CD116_08940, ERS140147_00900, NCTC13712_01668, rpmI
Sequence domains: Ribosomal protein L35
Large ribosomal subunit protein bL36 Chain: 4
Molecule details ›
Chain: 4
Length: 37 amino acids
Theoretical weight: 4.32 KDa
Source organism: Staphylococcus aureus
UniProt:
  • Canonical: A0A077UGV8 (Residues: 1-37; Coverage: 100%)
Gene names: CD116_01915, ERS140147_00615, NCTC13712_02175, rpmJ
Sequence domains: Ribosomal protein L36
Small ribosomal subunit protein uS2 Chain: b
Molecule details ›
Chain: b
Length: 226 amino acids
Theoretical weight: 25.93 KDa
Source organism: Staphylococcus aureus
UniProt:
  • Canonical: A0A2X2M020 (Residues: 1-226; Coverage: 97%)
Gene names: NCTC7878_01855, rpsB
Sequence domains: Ribosomal protein S2
Small ribosomal subunit protein uS3 Chain: c
Molecule details ›
Chain: c
Length: 202 amino acids
Theoretical weight: 22.68 KDa
Source organism: Staphylococcus aureus
UniProt:
  • Canonical: A0A077UU26 (Residues: 2-203; Coverage: 93%)
Gene names: CD116_01830, ERS140147_00632, NCTC13712_02192, rpsC
Sequence domains:
Small ribosomal subunit protein bS6 Chain: f
Molecule details ›
Chain: f
Length: 95 amino acids
Theoretical weight: 11.24 KDa
Source organism: Staphylococcus aureus
UniProt:
  • Canonical: W8TPC6 (Residues: 1-95; Coverage: 97%)
Gene names: CD116_05330, ERS140147_02571, NCTC13712_00312, rpsF
Sequence domains: Ribosomal protein S6
Small ribosomal subunit protein uS7 Chain: g
Molecule details ›
Chain: g
Length: 152 amino acids
Theoretical weight: 17.18 KDa
Source organism: Staphylococcus aureus
UniProt:
  • Canonical: A0A077VHU6 (Residues: 1-152; Coverage: 97%)
Gene names: CD116_12315, ERS140147_01024, NCTC13712_00499, rpsG
Sequence domains: Ribosomal protein S7p/S5e
Small ribosomal subunit protein uS8 Chain: h
Molecule details ›
Chain: h
Length: 131 amino acids
Theoretical weight: 14.72 KDa
Source organism: Staphylococcus aureus
UniProt:
  • Canonical: W8U8T8 (Residues: 2-132; Coverage: 99%)
Gene names: CD116_01870, ERS140147_00624, NCTC13712_02184, rpsH
Sequence domains: Ribosomal protein S8
Small ribosomal subunit protein uS9 Chain: i
Molecule details ›
Chain: i
Length: 127 amino acids
Theoretical weight: 14.31 KDa
Source organism: Staphylococcus aureus
UniProt:
  • Canonical: A0A1Q4GY48 (Residues: 4-130; Coverage: 98%)
Gene names: ACR74_08135, AS572_10930, E1948_03180, EP54_02240, EQ90_01125, GZ111_010295, LB359_05895, QU38_03565, rpsI
Sequence domains: Ribosomal protein S9/S16
Small ribosomal subunit protein uS10 Chain: j
Molecule details ›
Chain: j
Length: 97 amino acids
Theoretical weight: 11.01 KDa
Source organism: Staphylococcus aureus
UniProt:
  • Canonical: A0A0H2K0A0 (Residues: 6-102; Coverage: 95%)
Gene names: CD116_01795, ERS140147_00639, NCTC13712_02199, rpsJ
Sequence domains: Ribosomal protein S10p/S20e
Small ribosomal subunit protein uS11 Chain: k
Molecule details ›
Chain: k
Length: 114 amino acids
Theoretical weight: 12.05 KDa
Source organism: Staphylococcus aureus
UniProt:
  • Canonical: A0A077UKD6 (Residues: 15-128; Coverage: 88%)
Gene names: CD116_01925, ERS140147_00613, NCTC13712_02173, rpsK
Sequence domains: Ribosomal protein S11
30S ribosomal protein S12 Chain: l
Molecule details ›
Chain: l
Length: 135 amino acids
Theoretical weight: 15.08 KDa
Source organism: Staphylococcus aureus
Small ribosomal subunit protein uS14 Chain: n
Molecule details ›
Chain: n
Length: 60 amino acids
Theoretical weight: 7.19 KDa
Source organism: Staphylococcus aureus
UniProt:
  • Canonical: A0A077UGW7 (Residues: 2-61; Coverage: 98%)
Gene names: CD116_01865, ERS140147_00625, NCTC13712_02185, rpsN, rpsN1, rpsN_1, rpsZ
Sequence domains: Ribosomal protein S14p/S29e
Small ribosomal subunit protein uS17 Chain: q
Molecule details ›
Chain: q
Length: 80 amino acids
Theoretical weight: 9.37 KDa
Source organism: Staphylococcus aureus
UniProt:
  • Canonical: A0A077VLD5 (Residues: 5-84; Coverage: 92%)
Gene names: CD116_01845, ERS140147_00629, NCTC13712_02189, rpsQ
Sequence domains: Ribosomal protein S17
Small ribosomal subunit protein bS18 Chain: r
Molecule details ›
Chain: r
Length: 54 amino acids
Theoretical weight: 6.4 KDa
Source organism: Staphylococcus aureus
UniProt:
  • Canonical: A0A077UZF4 (Residues: 24-77; Coverage: 68%)
Gene names: CD116_05340, ERS140147_02569, NCTC13712_00314, rpsR
Sequence domains: Ribosomal protein S18
30S ribosomal protein S19 Chain: s
Molecule details ›
Chain: s
Length: 80 amino acids
Theoretical weight: 9.21 KDa
Source organism: Staphylococcus aureus
30S ribosomal protein S21 Chain: u
Molecule details ›
Chain: u
Length: 52 amino acids
Theoretical weight: 6.24 KDa
Source organism: Staphylococcus aureus
23S ribosomal RNA Chain: A
Molecule details ›
Chain: A
Length: 2905 nucleotides
Theoretical weight: 940.98 KDa
Sequence domains: Bacterial large subunit ribosomal RNA
5S ribosomal RNA Chain: B
Molecule details ›
Chain: B
Length: 115 nucleotides
Theoretical weight: 36.97 KDa
Sequence domains: 5S ribosomal RNA
16S ribosomal RNA Chain: a
Molecule details ›
Chain: a
Length: 1539 nucleotides
Theoretical weight: 498.06 KDa
Sequence domains: Bacterial small subunit ribosomal RNA

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.58Å
Relevant EMDB volumes: EMD-10079